Phosphoenolpyruvate → L-Glutamic Acid

Reaction equation

C([O-])(=O)CC(CC([O-])=O)(C([O-])=O)O.C=C(OP(O)(O)=O)C(O)=O.[NH2:24][C@H:25]([C:31]([O-:33])=[O:32])[CH2:26][CH2:27][C:28]([O-:30])=[O:29]>>[NH2:24][C@H:25]([C:31]([OH:33])=[O:32])[CH2:26][CH2:27][C:28]([OH:30])=[O:29]

Participants

Reactant 3

HCID 1005Phosphoenolpyruvate

HCID 31348Citrate

HCID 5460544L-glutamate(2-)

Product 1

HCID 33032L-Glutamic Acid

Procedure

Subsequently, a citrate synthase gene, a phosphoenolpyruvate carboxylase gene and a glutamate dehydrogenase gene derived from Escherichia coli were introduced into the SC17sucA strain.

Workup

additionwere introduced into the SC17sucA strain

Sources & evidence

ORD record: ord-ec5a45679f67495eab35811709f4f2aa
Source dataset: uspto-grants-2011_05
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: USRE042350E1

Reaction notes

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