N-Hydroxysuccinimido-d-biotin → Biotin

HRID 653385Notes

Reaction equation

HRID 653385 reaction equation
O[N:2]1[C:23](=[O:24])[NH:22][C@H:21]2[C@@H:3]1[CH2:4][S:5][C@H:6]2[CH2:7][CH2:8][CH2:9][CH:10](N1C(=O)CCC1=O)[C:11](=[O:13])[OH:12]>CS(C)=O>[OH:13][C:11]([CH2:10][CH2:9][CH2:8][CH2:7][C@H:6]1[C@@H:21]2[C@@H:3]([NH:2][C:23]([NH:22]2)=[O:24])[CH2:4][S:5]1)=[O:12]

Participants

3

Reactant 1

HCID 129676281N-Hydroxysuccinimido-d-biotin

Product 1

HCID 171548Biotin

Reagents, catalysts & solvents 1

HCID 679Dimethyl SulfoxideSolvent

Procedure

Dissolve 1 mg of N-hydroxysuccinimidobiotin (bio-NHS) in 1 ml dimethylsulfoxide. Add 7 ul of bio-NHS solution to protein (a 3 fold molar excess). Let sit 1 hr at room temperature. Dialyze vs PBS.

Workup

  1. customDialyze vs PBS

Sources & evidence

ORD record
ord-460124e2cad44e45925ff2c99d38b852
Source dataset
uspto-grants-1987_06
Patent number
US04676980

Reaction notes

Loading…