Monobasic potassium phosphate → Ribavirin
Reaction equation
[NH:1]1[CH:5]=[N:4][C:3]([C:6]([NH2:8])=[O:7])=[N:2]1.[C@@H:9]1(N2C3N=CN=C(O)C=3N=C2)[O:17][C@H:14]([CH2:15][OH:16])[C@@H:12]([OH:13])[C@H:10]1[OH:11].P([O-])(O)(O)=O.[K+]>>[CH:5]1[N:1]([C@@H:9]2[O:17][C@H:14]([CH2:15][OH:16])[C@@H:12]([OH:13])[C@H:10]2[OH:11])[N:2]=[C:3]([C:6]([NH2:8])=[O:7])[N:4]=1Participants
4
Procedure
Each 10 ml of the above suspensions of the treated microbial cells and 10 ml of a suspension of untreated microbial cells obtained similarly as in Example 1 was admixed with each 10 ml of a reaction substrate solution (pH 7.0) containing 20 mM 1,2,4-triazole-3-carboxamide, 20 mM inosine and 25 mM monopotassium dihydrogen phosphate, and the reaction was carried out at 60° C. for 24 hours. Analysis of ribavirin yields gave the results as shown in Table 4.