Reaction equation
B([O-])([O-])[O-].C(N(CC(O)=O)CC(O)=O)CN(CC(O)=O)CC(O)=O.Cl.C[O:27][C:28](=O)[C@H:29]([CH3:31])[NH2:30].[NH2:33][C@H:34]([C:40]([OH:42])=[O:41])[CH2:35][CH2:36][C:37](=[O:39])[NH2:38]>>[NH2:30][C@H:29]([C:28]([NH:33][C@H:34]([C:40]([OH:42])=[O:41])[CH2:35][CH2:36][C:37](=[O:39])[NH2:38])=[O:27])[CH3:31]Participants
5
Procedure
Each strain of the microbial cells thus obtained was recovered from the agar media, and suspended in a 0.1 M borate buffer (pH 9.0) containing 10 mM EDTA so as to prepare a suspension containing 100 g/l of wet microbial cells. 0.1 ml of each microbial cell suspension was admixed with 0.1 ml of 100 mM borate buffer (pH 9.0) containing 10 mM EDTA, 200 mM L-alanine methyl ester hydrochloride and 400 mM L-glutamine to prepare a total volume 0.2 ml of the reaction mixture, which was then subjected to the reaction at 20° C. for the time period shown in Table 1. The amount (mM) of L-alanyl-L-glutamine (Ala-Gin) produced by each reaction is shown in Table 1.
Workup
- customEach strain of the microbial cells thus obtained
- customwas recovered from the agar media
- additioncontaining 10 mM EDTA so as
- customto prepare a suspension
- additioncontaining 100 g/l of wet microbial cells
- customto prepare a total volume 0.2 ml of the reaction mixture, which
- customwas then subjected to the reaction at 20° C. for the time period
Sources & evidence
- ORD record
- ord-2dcdcaae011e43918232a6dec5eab105
- Source dataset
- uspto-grants-2011_12
- Patent number
- US08084227B2
Linked notes
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