Panose → Isomaltose

HRID 734172

Reaction equation

HRID 734172 reaction equation
[CH2:1]([OH:34])[C@H:2]1[O:7][C@H:6]([O:8][CH2:9][C@H:10]2[O:15][C@H:14]([O:16][C@@H]([C@H](O)[C@@H](O)C=O)[C@H](O)CO)[C@H:13]([OH:28])[C@@H:12]([OH:29])[C@@H:11]2[OH:30])[C@H:5]([OH:31])[C@@H:4]([OH:32])[C@@H:3]1[OH:33]>O>[CH2:1]([OH:34])[C@H:2]1[O:7][C@H:6]([O:8][CH2:9][C@H:10]2[O:15][CH:14]([OH:16])[C@H:13]([OH:28])[C@@H:12]([OH:29])[C@@H:11]2[OH:30])[C@H:5]([OH:31])[C@@H:4]([OH:32])[C@@H:3]1[OH:33]

Participants

3

Reactant 1

HCID 94448Panose

Product 1

HCID 439193Isomaltose

Reagents, catalysts & solvents 1

HCID 962WaterSolvent

Conditions

Temperature
100 °C

Procedure

To a 0.2% aqueous solution of panose, α-isomaltosylmaltose, α-isomaltosyltriose, α-isomaltosyltetraose, or cyclotetrasaccharide was added 100 units/g solid, d.s.b., of an isomaltodextranase specimen, obtained by the method in Experiment 29, where 3,000 units/g solid, d.s.b., of the specimen was also used for the aqueous solution with cyclotetrasaccharide. The mixture was subjected to an enzymatic reaction at 40° C. and pH 5.5 for 24 hours and heated at 100° C. for 20 min to suspend the enzymatic reaction. The saccharide composition of the resulting mixture was analyzed on HPLC using column of “MCIGEL CK04SS”, a column commercialized by Mitsubishi Chemical Industries, Ltd., Tokyo, Japan; an inner column temperature of 80° C.; a flow rate of 0.5 ml/min of water as an eluate; and a detector of “RI-8012”, a differential refractometer commercialized by Tosoh Corporation, Tokyo, Japan. The results are in Table 34.

Workup

  1. custom, of an isomaltodextranase specimen, obtained by the method in Experiment 29, where 3,000 units/g solid
  2. customThe mixture was subjected to an enzymatic reaction at 40° C.
  3. customthe enzymatic reaction
  4. customan inner column temperature of 80° C.

Sources & evidence

ORD record
ord-116ecd8308bc43d79b4c552e4c31e063
Source dataset
uspto-grants-2009_09
Patent number
US07592160B2

Linked notes

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