Reaction equation
[CH3:1][C@@H:2]1O[C@@H:5]([O:7][C@H:8]2[C@H:13]([OH:14])[C@@H:12](O)[C@H:11](NC(N)=N)[C@@H:10](O)[C@@H:9]2NC(N)=N)[C@H:4]([O:25][C@@H]2O[C@@H](CO)[C@H](O)[C@@H](O)[C@@H]2NC)[C@@:3]1(O)C=O.C[C:42]1(C)S[C@@H:45]2[C@H:47](NC(CC3C=CC=CC=3)=O)[C:48](=O)[N:44]2[C@H:43]1C([O-])=O.[K+].[C:65](=O)=O>>[CH:2]1[C:1]2[CH2:42][C@H:43]3[N:44]([CH2:45][CH2:47][C@@:9]45[C@H:10]3[CH:11]=[CH:12][C@H:13]([OH:14])[C@@H:8]4[O:7][C:5]([C:65]=25)=[C:4]([OH:25])[CH:3]=1)[CH3:48]Participants
4
Conditions
- Temperature
- 37 °C
Procedure
Human neuroblastoma SH-SY5Y cells were maintained as a monolayer culture in Dulbecco's modified eagle medium (DMEM) supplemented with 10% fetal calf serum, 100 μg/ml streptomycin and 100 units/ml penicillin in 5% carbon dioxide, incubator maintained at 37° C. (Wang et al., 2000). Cells were plated into flasks a week before experiments Treatments were terminated at the designated times by replacing the medium with ice-cold phosphate buffered saline (PBS) on ice and subsequently rinsed with PBS three times. Cells then were used for cAMP assay or harvested as described in Section C10
Workup
- temperatureHuman neuroblastoma SH-SY5Y cells were maintained as a monolayer culture in Dulbecco's
- waitCells were plated into flasks a week before experiments Treatments
- customwere terminated at the designated times
- washsubsequently rinsed with PBS three times
Sources & evidence
- ORD record
- ord-5cd557340ebd447090a4e7284c94d7c7
- Source dataset
- uspto-grants-2007_08
- Patent number
- US07256200B2
Linked notes
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