Mannitol → Epothilone D Hydroxypropyl-beta-Cyclodextrin

Participants

Reaction Steps

A combination of ten milligrams (“mg”) of epothilone D and 0.4 grams (“g”) of hydroxypropyl-β-cyclodextrin (“HPβCD”) were dissolved in 60% tert-butanol-water to make 1 milliliter (“mL”) of solution. A second solution having ten mg of epothilone D and ten mg of mannitol dissolved in 60% tert-butanol-water was prepared. A third solution of ten mg of epothilone D and ten mg of mannitol in 60% tert-butanol-water was also prepared. Formulation solutions containing ten mg/mL epothilone D were poured into 8 mL glass vials for lyophilization.

Provenance & Evidence