Sodium Hydroxide → L-Lysine

Temperature

110 °C

20.88 Grams (90 mmol) of L-lysine methyl ester dihydrochloride was dissolved in 18 ml of methanol, and 45 ml of 2 N aqueous sodium hydroxide solution was added thereto to prepare a treating agent solution. An untreated cotton cloth (480 mm×510 mm, 45 g) was allowed to uniformly absorb the total amount of the above treating agent solution and then air-dried for 2 hours at room temperature. The dried cloth was heat-treated for 20 minutes at 140° C. in a batch type hot air oven (“IPHH-2000”, an inert oven manufactured by Tabaiesbeck Co., Ltd.) and washed with water. It was further washed with 5% aqueous sodium bicarbonate solution and rinsed three times with water and subsequently was washed with 10% aqueous citric acid solution and rinsed three times with water, and finally washed with household washing detergent solution, rinsed with water and air-dried. A part of the cloth was cut and a solution of ninhydrin in butanol was sprayed on it. Thereafter, it was heated for 10 minutes in an oven of 110° C. to cause coloring thereby the presence of the amino acid was confirmed. Also, a part of the cloth was cut and subjected to an infrared spectroscopic analysis whereby there was confirmed a peak based on ester bond at 1742 cm−1. As a result of determination for the amount of the L-lysine bonded, it was 0.10 mmol based on 1 g of the cloth. The degree of esterfication was 0.0 16.

1. customto prepare a treating agent solution
2. customto uniformly absorb the total amount of the above treating agent solution
3. customair-dried for 2 hours at room temperature
4. additionThe dried cloth was heat-treated for 20 minutes at 140° C. in a batch type hot air oven (“IPHH-2000”, an inert oven manufactured by Tabaiesbeck Co., Ltd.)
5. washwashed with water
6. washIt was further washed with 5% aqueous sodium bicarbonate solution
7. washrinsed three times with water
8. washsubsequently was washed with 10% aqueous citric acid solution
9. washrinsed three times with water
10. washfinally washed with household
11. washwashing detergent solution
12. washrinsed with water
13. customair-dried

ORD record

ord-fd57e21a6052402ba92034b341776a35

Source dataset

uspto-grants-2006_08

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US07091221B2