Sodium Hydroxide → L-Lysine

Temperature

110 °C

2.62 Grams (10 mmol) of L-lysine n-propyl ester dihydrochloride was dissolved in 2 ml of methanol, and 5 ml of 2 N aqueous sodium hydroxide solution was added thereto to prepare a treating agent solution. An untreated cotton cloth (160 mm×170 mm, 5.0 g) was allowed to uniformly absorb the total amount of the above treating agent solution and then air-dried for an hour and 15 minutes at room temperature. The dried cloth was heat-treated for 20 minutes at 140° C. in a batch type hot air oven (“IPHH-2000”, an inert oven manufactured by Tabaiesbeck Co., Ltd.) and washed with water. It was further washed with 5% aqueous sodium bicarbonate solution and rinsed three times with water and subsequently was washed with 10% aqueous citric acid solution and rinsed three times with water, and finally washed with household washing detergent solution, rinsed with water and air-dried. A part of the cloth was cut and a solution of ninhydrin in butanol was sprayed on it. Thereafter, it was heated for 10 minutes in an oven of 110° C. to cause coloring thereby the presence of the amino acid was confirmed. As a result of determination for the amount of the bonded L-lysine, it was 0.08 mmol based on 1 g of the cloth. The degree of esterfication was 0.013.

1. customto prepare a treating agent solution
2. customto uniformly absorb the total amount of the above treating agent solution
3. customair-dried for an hour and 15 minutes at room temperature
4. additionThe dried cloth was heat-treated for 20 minutes at 140° C. in a batch type hot air oven (“IPHH-2000”, an inert oven manufactured by Tabaiesbeck Co., Ltd.)
5. washwashed with water
6. washIt was further washed with 5% aqueous sodium bicarbonate solution
7. washrinsed three times with water
8. washsubsequently was washed with 10% aqueous citric acid solution
9. washrinsed three times with water
10. washfinally washed with household
11. washwashing detergent solution
12. washrinsed with water
13. customair-dried

ORD record

ord-19b3a387dab34adbb5e6b6e4e17962c8

Source dataset

uspto-grants-2006_08

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US07091221B2