Phosphate → 4-Nitrophenol
Reaction equation
[CH:1]1[C:6]([N+:7]([O-:9])=[O:8])=[CH:5][CH:4]=[C:3]([O:10][C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[CH:2]=1.P([O-])([O-])([O-])=O>>[N+:7]([C:6]1[CH:1]=[CH:2][C:3]([OH:10])=[CH:4][CH:5]=1)([O-:9])=[O:8]Participants
3
Conditions
- Temperature
- 37 °C
Procedure
With 0.5 ml of a substrate solution containing 20 mM 4-nitrophenyl-α-D-glucopyranoside was mixed 1.0 ml of 0.1M phosphate buffer (pH 7.0), and the mixture was pre-heated at 37° C. for 5 minutes. Thereafter, 0.5 ml of an enzyme solution was added thereto and the reaction was conducted at 37° C. for 5 minutes, followed by determination of changes in absorbance per minute at 400 nm. The enzyme activity producing 1 μmol of 4-nitrophenol per minute under the above-mentioned conditions was taken as one unit.
Sources & evidence
- ORD record
- ord-38f0e029642a405aaf33735e4cd2892c
- Source dataset
- uspto-grants-1997_03
- Patent number
- US05607838
Reaction notes
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