アスコルビン酸オキシダ-ゼ
アスコルビン酸オキシダ-ゼ · アスコルビン酸オキシダーゼ · アスコルビン酸オキシダーゼ FROM CUCURBITA SP. · ASO · AOX · Ascorbate Oxidase from microorganism · ASOD · C:C&B8
識別子と物理属性
| CAS No. | 9029-44-1 |
|---|---|
| 分子式 | C35H36N6O3 |
| 平均分子量 | 588.7 g/mol |
| 単同位体質量 | 588.28488903 g/mol |
| SMILES | Cc1c2n(C)c3ccc(cc13)CCC=CCCn1nnc3c(C)c(cnc31)C(CC(=O)O)c1ccc3c(c1)CN(CC3)C2=O |
| InChIKey | IJDQXSQWJLQCRL-UHFFFAOYSA-N |
| PubChem CID | 168009863 |
| EC No. | 232-852-6 |
| 現地名 | アスコルビン酸オキシダ-ゼ |
化学・物理的性質
| XLogP | 5.1 |
|---|---|
| 極性表面積 | 106 |
| 水素結合供与体数 | 1 |
| 水素結合受容体数 | 6 |
| 回転可能結合数 | 2 |
| 重原子数 | 44 |
| 形式電荷 | 0 |
| 複雑度 | 1,090 |
| 貯蔵条件 | 2-8°C |
| 溶解度 | 水性緩衝液に可溶。 |
| 外観・状態 | 粉 |
| 色 | かすかに青みがかった |
| 由来生物 | plant (Cucurbita spp.) |
| Specific Activity | 1,000-3,000units/mg protein |
化学的特性
Appearance : Light blue amorphous powder, lyophilized Stability : Store at －20℃ (A decrease in activity of ca.20% may occur at 5℃ within 6 months.) Molecular weight : 132,000, 140,000 Isoelectric point : between 6.0 and 7.8 , 8.2 Michaelis constant : 2.5×10－4M (Ascorbate) Structure : 8 copper atoms per mol of enzyme Inhibitors : cyanide, Na2S, diethyldithiocarbamate (Na) Optimum pH : 5.6 Optimum temperature : approx. 30℃ pH Stability : pH 7.0－10.0 (25℃, 17hr) Thermal stability : below 40℃ (pH 8.0, 30min) Substrate specificity : This enzyme oxidizes ascorbic acid and several ascorbic derivatives.
安全性と法規制
| GHS 分類 | This chemical does not meet GHS hazard criteria for 14.3% (1 of 7) of reports.DangerH334 (85.7%): May cause allergy or asthma symptoms or breathing difficulties if inhaled [Danger Sensitization, respiratory]P233, P260, P271, P284, P304+P340, P342+P316, P403, and P501 (click each P-code to see the statement)Aggregated GHS information provided per 7 reports by companies from 2 notifications to the ECHA C&L Inventory. |
|---|---|
| 規制情報 | Oxidase, ascorbate: Does not have an individual approval but may be used under an appropriate group standard |
| Hazard Classes and Categories | Resp. Sens. 1 (85.7%) |
| F | 10-21 |
| 水環境危害等級（WGK） | 3 |
| 税関コード | 35079090 |
| 安全措置（S） | 24/25 |
| 危険等級 | Resp. Sens. 1 |
| 貯蔵分類 | 11 - Combustible Solids |
用途
用途
過酸化水素の定量系においてアスコルビン酸の干渉除去に用いる。
使用
Ascorbate oxidase, from Cucurbita sp., may be used to study oxidative stress and heat stress response and tolerance. Also used in ascorbic acid assays to study the heat stress response of Arabidopsis and useful for enzymatic determination of ascorbic acid and for eliminating the interference of ascorbic acid in clinical analysis.
一般的な説明
Ascorbate oxidase is a homodimeric enzyme, which comprises 552 amino acid residues per subunit (zucchini). It corresponds to a molecular mass of 70kDa per subunit. This enzyme is mainly found in plants, fungi and eubacteria.