アンジオテンシン変換酵素 ウサギ肺由来

使用Angiotensin converting enzyme from rabbit lung has been used:for measuring inhibitory effect of egg white protein hydrolysates on ACE activity by high performance liquid chromatography (HPLC)to measure the ACE inhibition by litchi pericarp and cooked chicken breast using hippuryl-L-histidyl-L-leucine (HHL) as substrate by reverse phase-HPLC (RP-HPLC)3 and HPLC respectively in releasing GPI anchored protein in vitro in few cell lines like HeLa, HEK293 and in vivo in mice sperm.

一般的な説明The angiotensin-converting enzyme (ACE) is a dipeptidyl-carboxypeptidase which exists in somatic and testicular isoforms with zinc binding motif HEXXH in their active site. ACE regulates blood pressure through renin-angiotensin system. ACE elevates blood pressure by converting angiotensin I to a key vasoconstrictor angiotensin II and inhibiting a potent vasodilator bradykinin. Inhibition of ACE is a targeted therapeutic strategy for high blood pressure. Several ACE synthetic inhibitory peptides available for clinical use include captopril, enalapril and lisinopril. Currently, developing inhibitory peptides from natural food sources, or phenolic compounds from plant sources to inhibit ACE is underway. ACE plays a critical role in fertilization by releasing the proteins anchored to glycosylphosphatidylinositol (GPI) in sperm membrane.

純化方法Purify ACE by fractionation on DEAE-cellulose, Ca phosphate gel chromatography, elution from Sephadex G-200 and lectin affinity chromatography. The MW varied with glycosidation and is higher by gel filtration. It contains one atom of Zn/mol and has Km values for hydrolysis of hippurylhistidinylleucine and angiotensin I of 2.3 and 0.07 mM, and turnover of 15,430 and 792 mol/min/mol at 37o, respectively. The activity is inhibited by EDTA and increased amounts of Ca ions but required Ca ions. [Das & Soffer J Biol Chem 250 6762 1975, Reviewed by Ehlers & Riordan Biochemistry 28 5311 1989.]