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HCID 4156作成日：2026年10月27日

メタンスルホン酸メチル

メタンスルホン酸メチル · as-ジメチルスルファイト · メチル=メタンスルホナート · メチルメタンスルホナート · メタノールメタンスルホナート · メチルメシラート · メチルメタンスルホン酸 · メチルメタン硫酸塩

https://huagongshe.com/ja/chemical/4156

メタンスルホン酸メチル の分子構造式

識別子と物理属性

CAS No.66-27-3
分子式C2H6O3S
平均分子量110.13 g/mol
単同位体質量110.00376505 g/mol
SMILESCOS(C)(=O)=O
InChIKeyMBABOKRGFJTBAE-UHFFFAOYSA-N
PubChem CID4156
ChEMBLCHEMBL313493
ChEBICHEBI:25255
EC No.200-625-0
DTXSIDDTXSID7020845
現地名メタンスルホン酸メチル

化学・物理的性質

XLogP-0.4
極性表面積51.8
水素結合供与体数0
水素結合受容体数3
回転可能結合数1
重原子数6
形式電荷0
複雑度105
物質分類Other Classes -> Sulfur Compounds
SpringerMaterials PropertiesChemical shift
密度1.2943 at 68 °F (NTP, 1992) - Denser than waterwill sink1.2943 at 20 °C/4 °C1.3 g/mL at 25 °C(lit.)
Color/FormColorless liquid
溶解度greater than or equal to 100 mg/mL at 73 °F (NTP, 1992)Solubility in water about 1:5slightly sol in nonpolar solventsSol in dimethyl formamide and in propylene glycol (1 in 1)200g/リットル200g/L(20℃)
引火点220 °F (NTP, 1992)
沸点396 to 397 °F at 760 mmHg (NTP, 1992)203 °C at 753 mm Hg202-203 °C(lit.)
融点68 °F (NTP, 1992)20 °C20°C
蒸気圧0.31 [mmHg]0.8 hPa (59 °C)
屈折率Index of refraction 1.4140 at 20 °C/Dn20/D 1.414(lit.)
Physical DescriptionMethyl methanesulfonate is a colorless to amber liquid. (NTP, 1992)Colorless liquidmp = 20 deg C[HSDB] Colorless to amber liquid[CAMEO] Clear faintly yellow liquid[MSDSonline]
Other Experimental Properties... decompose on distillation at normal pressure ... darken when stored in the absence of light ... form hydrates, are hygroscopic, and dissolve readily in water /Alkanesulfonic acids/
貯蔵条件Store below +30°C.
外観・状態液体
色無色～淡黄色
Merck14,6097
BRN1098586
ヘンリー定数2.5×100 mol/(m3Pa) at 25℃, HSDB (2015)
安定性安定。可燃性。強酸化剤、強酸、強塩基とは相容れない。
InChI1S/C2H6O3S/c1-5-6(2,3)4/h1-2H3
InChIKeyMBABOKRGFJTBAE-UHFFFAOYSA-N
SMILESCOS(C)(=O)=O
CAS データベース66-27-3(CAS DataBase Reference)
NISTの化学物質情報Methanesulfonic acid, methyl ester(66-27-3)
EPAの化学物質情報Methyl methanesulfonate (66-27-3)

化学的特性

colourless liquid

安全性と法規制

GHS 分類DangerH301 (99.5%): Toxic if swallowed [Danger Acute toxicity, oral]P203, P260, P261, P264, P264+P265, P270, P271, P272, P273, P280, P301+P316, P302+P352, P304+P340, P305+P351+P338, P318, P319, P321, P330, P332+P317, P333+P317, P337+P317, P362+P364, P391, P403+P233, P405, and P501 (click each P-code to see the statement)Aggregated GHS information provided per 221 reports by companies from 11 notifications to the ECHA C&L Inventory. Each notification may be associated with multiple companies.H301: Toxic if swallowed [Danger Acute toxicity, oral]
規制情報Chemical: Methanesulfonic acid, methyl esterHazard Traits - CarcinogenicityStatus: Active Update: 30-06-2022 https://echa.europa.eu/registration-dossier/-/registered-dossier/17543Methyl methanesulphonate: Does not have an individual approval but may be used under an appropriate group standard
その他の安全情報IMAP assessments - Methanesulfonic acid, methyl ester: Human health tier I assessment
輸送ラベル（DOT）Corrosive Poison
火災危険This chemical is combustible. (NTP, 1992)
健康有害性SYMPTOMS: Symptoms of exposure to this compound may include irritation of the eyes, skin, mucous membranes, nose and respiratory tract. It can cause dermatitis and nausea. Large doses ingested over nearly a year produced gastrointestinal and hepatic toxic effects.
有害性の概要At a dose of 2.8-800 mg/kg body weight for up to 350 days, side effects in cancer patients included gastrointestinal and liver injury[HSDB] Embryotoxic at 100 mg/kg in ratsNo human data[REPROTOX] May cause skin, eye, mucous membrane, and respiratory tract irritation[CAMEO] An irritant[MSDSonline]
反応性グループSulfonates, Phosphonates, and Thiophosphonates, Organic
Special ReportsNational Toxicology Program. Eleventh Report on Carcinogens (2005). The Report on Carcinogens is an informational scientific and public health document that identifies and discusses substances (including agents, mixtures, or exposure circumstances) that may pose a carcinogenic hazard to human health. Methyl Methanesulfonate (66-27-3) is listed as reasonably anticipated to be a human carcinogen.[Available from, as of July 31, 2009: http://ntp.niehs.nih.gov/ntp/roc/eleventh/profiles/s110meme.pdf]

用途

用途

重合反応触媒、アルキル化及びエステル化試薬

使用

Methyl methanesulfonate is a DNA adduct that adds methyl groups to Dan at 7-guanine, 3-guanine and 3-adenine.

一般的な説明

Colorless to amber liquid.

製法・合成法

純化方法

Purify the ester by careful fractionation and collecting the middle fraction. Suspected CARCINOGEN. Note that MeSO3H has b 167-167.5o/10mm and methanesulfonic anhydride has b 138o/10mm)—both are possible impurities. [Beilstein 4 IV 11.]

Reactivity ProfileMETHYL METHANESULFONATE is incompatible with strong oxidizing agents, strong acids and strong bases. (NTP, 1992)
Air and Water ReactionsWater soluble.
Hazard Classes and CategoriesAcute Tox. 3 (99.5%)Acute toxicity (Oral) - Category 3
Shipment Methods and RegulationsPRECAUTIONS FOR "CARCINOGENS": Procurement ... of unduly large amt ... should be avoided. To avoid spilling, carcinogens should be transported in securely sealed glass bottles or ampoules, which should themselves be placed inside strong screw-cap or snap-top container that will not open when dropped & will resist attack from the carcinogen. Both bottle & the outside container should be appropriately labelled. ... National post offices, railway companies, road haulage companies & airlines have regulations governing transport of hazardous materials. These authorities should be consulted before ... material is shipped. /Chemical Carcinogens/PRECAUTIONS FOR "CARCINOGENS": When no regulations exist, the following procedure must be adopted. The carcinogen should be enclosed in a securely sealed, watertight container (primary container), which should be enclosed in a second, unbreakable, leakproof container that will withstand chem attack from the carcinogen (secondary container). The space between primary & secondary container should be filled with absorbent material, which would withstand chem attack from the carcinogen & is sufficient to absorb the entire contents of the primary container in the event of breakage or leakage. Each secondary container should then be enclosed in a strong outer box. The space between the secondary container & the outer box should be filled with an appropriate quantity of shock-absorbent material. Sender should use fastest & most secure form of transport & notify recipient of its departure. If parcel is not received when expected, carrier should be informed so that immediate effort can be made to find it. Traffic schedules should be consulted to avoid ... arrival on weekend or holiday ... /Chemical Carcinogens/
消火Fires involving this material can be controlled with a dry chemical, carbon dioxide or Halon extinguisher. (NTP, 1992)
応急処置EYES: First check the victim for contact lenses and remove if present. Flush victim's eyes with water or normal saline solution for 20 to 30 minutes while simultaneously calling a hospital or poison control center. Do not put any ointments, oils, or medication in the victim's eyes without specific instructions from a physician. IMMEDIATELY transport the victim after flushing eyes to a hospital even if no symptoms (such as redness or irritation) develop.
保管条件PRECAUTIONS FOR "CARCINOGENS": Storage site should be as close as practical to lab in which carcinogens are to be used, so that only small quantities required for ... expt need to be carried. Carcinogens should be kept in only one section of cupboard, an explosion-proof refrigerator or freezer (depending on chemicophysical properties ...) that bears appropriate label. An inventory ... should be kept, showing quantity of carcinogen & date it was acquired ... Facilities for dispensing ... should be contiguous to storage area. /Chemical Carcinogens/
清掃方法PRECAUTIONS FOR "CARCINOGENS": A high-efficiency particulate arrestor (HEPA) or charcoal filters can be used to minimize amt of carcinogen in exhausted air ventilated safety cabinets, lab hoods, glove boxes or animal rooms ... Filter housing that is designed so that used filters can be transferred into plastic bag without contaminating maintenance staff is avail commercially. Filters should be placed in plastic bags immediately after removal ... The plastic bag should be sealed immediately ... The sealed bag should be labelled properly ... Waste liquids ... should be placed or collected in proper containers for disposal. The lid should be secured & the bottles properly labelled. Once filled, bottles should be placed in plastic bag, so that outer surface ... is not contaminated ... The plastic bag should also be sealed & labelled. ... Broken glassware ... should be decontaminated by solvent extraction, by chemical destruction, or in specially designed incinerators. /Chemical Carcinogens/
非火災時の漏出対応SMALL SPILLS AND LEAKAGE: If you should spill this chemical, use absorbent paper to pick up all liquid spill material. Seal the absorbent paper, as well as any of your clothing which may be contaminated, in a vapor-tight plastic bag for eventual disposal. Wash any surfaces you may have contaminated with a soap and water solution. Do not reenter the contaminated area until the Safety Officer (or other responsible person) has verified that the area has been properly cleaned.
処分方法SRP: The most favorable course of action is to use an alternative chemical product with less inherent propensity for occupational exposure or environmental contamination. Recycle any unused portion of the material for its approved use or return it to the manufacturer or supplier. Ultimate disposal of the chemical must consider: the material's impact on air qualitypotential migration in soil or watereffects on animal, aquatic, and plant lifeand conformance with environmental and public health regulations.PRECAUTIONS FOR "CARCINOGENS": There is no universal method of disposal that has been proved satisfactory for all carcinogenic compounds & specific methods of chem destruction ... published have not been tested on all kinds of carcinogen-containing waste. ... summary of avail methods & recommendations ... /given/ must be treated as guide only. /Chemical Carcinogens/PRECAUTIONS FOR "CARCINOGENS": ... Incineration may be only feasible method for disposal of contaminated laboratory waste from biological expt. However, not all incinerators are suitable for this purpose. The most efficient type ... is probably the gas-fired type, in which a first-stage combustion with a less than stoichiometric air:fuel ratio is followed by a second stage with excess air. Some ... are designed to accept ... aqueous & organic-solvent solutions, otherwise it is necessary ... to absorb soln onto suitable combustible material, such as sawdust. Alternatively, chem destruction may be used, esp when small quantities ... are to be destroyed in laboratory. /Chemical Carcinogens/PRECAUTIONS FOR "CARCINOGENS": HEPA (high-efficiency particulate arrestor) filters ... can be disposed of by incineration. For spent charcoal filters, the adsorbed material can be stripped off at high temp & carcinogenic wastes generated by this treatment conducted to & burned in an incinerator. ... LIQUID WASTE: ... Disposal should be carried out by incineration at temp that ... ensure complete combustion. SOLID WASTE: Carcasses of lab animals, cage litter & misc solid wastes ... should be disposed of by incineration at temp high enough to ensure destruction of chem carcinogens or their metabolites. /Chemical Carcinogens/For more Disposal Methods (Complete) data for METHYL METHANESULFONATE (7 total), please visit the HSDB record page.
Preventive MeasuresPRECAUTIONS FOR "CARCINOGENS": Smoking, drinking, eating, storage of food or of food & beverage containers or utensils, & the application of cosmetics should be prohibited in any laboratory. All personnel should remove gloves, if worn, after completion of procedures in which carcinogens have been used. They should ... wash ... hands, preferably using dispensers of liq detergent, & rinse ... thoroughly. Consideration should be given to appropriate methods for cleaning the skin, depending on nature of the contaminant. No standard procedure can be recommended, but the use of organic solvents should be avoided. Safety pipettes should be used for all pipetting. /Chemical Carcinogens/PRECAUTIONS FOR "CARCINOGENS": In animal laboratory, personnel should remove their outdoor clothes & wear protective suits (preferably disposable, one-piece & close-fitting at ankles & wrists), gloves, hair covering & overshoes. ... Clothing should be changed daily but ... discarded immediately if obvious contamination occurs ... /also,/ workers should shower immediately. In chemical laboratory, gloves & gowns should always be worn ... however, gloves should not be assumed to provide full protection. Carefully fitted masks or respirators may be necessary when working with particulates or gases, & disposable plastic aprons might provide addnl protection. If gowns are of distinctive color, this is a reminder that they should not be worn outside of lab. /Chemical Carcinogens/PRECAUTIONS FOR "CARCINOGENS": ... Operations connected with synth & purification ... should be carried out under well-ventilated hood. Analytical procedures ... should be carried out with care & vapors evolved during ... procedures should be removed. ... Expert advice should be obtained before existing fume cupboards are used ... & when new fume cupboards are installed. It is desirable that there be means for decreasing the rate of air extraction, so that carcinogenic powders can be handled without ... powder being blown around the hood. Glove boxes should be kept under negative air pressure. Air changes should be adequate, so that concn of vapors of volatile carcinogens will not occur. /Chemical Carcinogens/PRECAUTIONS FOR "CARCINOGENS": Vertical laminar-flow biological safety cabinets may be used for containment of in vitro procedures ... provided that the exhaust air flow is sufficient to provide an inward air flow at the face opening of the cabinet, & contaminated air plenums that are under positive pressure are leak-tight. Horizontal laminar-flow hoods or safety cabinets, where filtered air is blown across the working area towards the operator, should never be used ... Each cabinet or fume cupboard to be used ... should be tested before work is begun (eg, with fume bomb) & label fixed to it, giving date of test & avg air-flow measured. This test should be repeated periodically & after any structural changes. /Chemical Carcinogens/For more Preventive Measures (Complete) data for METHYL METHANESULFONATE (9 total), please visit the HSDB record page.
Isolation and EvacuationExcerpt from ERG Guide 154 [Substances - Toxic and/or Corrosive (Non-Combustible)]:
Personal Protective Equipment (PPE)MINIMUM PROTECTIVE CLOTHING: If Tyvek-type disposable protective clothing is not worn during handling of this chemical, wear disposable Tyvek-type sleeves taped to your gloves.PRECAUTIONS FOR "CARCINOGENS": ... Dispensers of liq detergent /should be available./ ... Safety pipettes should be used for all pipetting. ... In animal laboratory, personnel should ... wear protective suits (preferably disposable, one-piece & close-fitting at ankles & wrists), gloves, hair covering & overshoes. ... In chemical laboratory, gloves & gowns should always be worn ... however, gloves should not be assumed to provide full protection. Carefully fitted masks or respirators may be necessary when working with particulates or gases, & disposable plastic aprons might provide addnl protection. ... Gowns ... /should be/ of distinctive color, this is a reminder that they are not to be worn outside the laboratory. /Chemical Carcinogens/
相互作用The combined effects of methyl methanesulfonate and ethyl methanesulfonate on the induction of 6-thioguanine resistant mutants and chromosome aberrations were examined in Chinese hamster V79 cells. Cells were simultaneously treated with ethylmethanesulfonate at a concentration of D20 /SRP: D20 = concentration required to reduce cell survival to 20%/ and methyl methanesulfonate at various concentrations for 3, 6 or 9 hr. In other experiments cells were simultaneously treated with methyl methanesulfonate at a concentration of D20 and ethyl methanesulfonate at various concentrations for 3, 6 or 9 hr. The mathematical analysis of the combined effects of both chemicals for cell killing (cytotoxicity) and 6-thioguanine resistant mutations indicates that synergistic interactions were observed for both cell killing and mutations induced by methyl methanesulfonate and ethyl methanesulfonate. The frequency of chromosome aberrations induced by simultaneous treatment with methyl methanesulfonate at a concentration of D20 and ethyl methanesulfonate at various concentrations for 3 hr was additive. However, the frequency of chromosome aberrations induced by ethyl methanesulfonate at a concentration of D20 and methyl methanesulfonate at various concentrations for 3 hr was not significantly different from those induced by methyl methanesulfonate alone.Ethanol itself did not induce any apparent chromosome aberrations in Chinese hamster ovary cells. However, post-treatment with ethanol potentiated the chromosome aberrations induced by ... methyl methanesulfonate. ... Chromatid exchanges were predominantly increased in cultures treated with ... methyl methanesulfonate ... and then with ethanol. ... Post-treatment with acetaldehyde, the major metabolite of ethanol, also potentiated the chromosome aberrations induced by ... methyl methanesulfonate. ... The main types of aberrations potentiated by posttreatment with acetaldehyde were similar to those by posttreatment with ethanol. /Methyl methanesulfonate/
生態毒性値EC50Species: Oryzias latipes (Medaka, high-eyes, embryo 66 hr postfertilization)Conditions: freshwater, static, 20 °CConcentration: 12.5 mmol/L for 2 hr (95% confidence interval: 10.9-14.0 mmol/L)Effect: growthLC50Concentration: 15.8 mmol/L for 2 hr (95% confidence interval: 14.0-17.8 mmol/L)LC50 Eisenia fetida (earthworm, mature, weight 370-450 mg) direct application 1-10 ug/sq cm for 48 hr
環境中動態TERRESTRIAL FATE: Based on a classification scheme(1), an estimated Koc value of 12(SRC), determined from a structure estimation method(2), indicates that methyl methanesulfonate is expected to have very high mobility in soil(SRC). Volatilization of methyl methanesulfonate from moist soil surfaces may occur SRC) given an estimated Henry's Law constant of 4.0X10-6 atm-cu m/mole(SRC), using a fragment constant estimation method(3). However, hydrolysis of methyl methanesulfonate is expected to be the major fate process in moist soil based on measured half-lives in water of 4.56 hours(5), 9.66 hours(6), and 77 hours(7). Methyl methanesulfonate is not expected to volatilize from dry soil surfaces(SRC) based upon an estimated vapor pressure of 0.3 mm Hg(SRC), determined from a fragment constant method(4). Biodegradation data were not available(SRC, 2007).AQUATIC FATE: Based on a classification scheme(1), an estimated Koc value of 12(SRC), determined from a structure estimation method(2), indicates that methyl methanesulfonate is not expected to adsorb to suspended solids and sediment(SRC). Volatilization from water surfaces is expected(3) based upon an estimated Henry's Law constant of 4.0X10-6 atm-cu m/mole(SRC), developed using a fragment constant estimation method(4). Using this Henry's Law constant and an estimation method(3), volatilization half-lives for a model river and model lake are 6 and 74 days, respectively(SRC). However, the rapid hydrolysis of methyl methanesulfonate, with half-lives of 4.56 hours(9), 9.66 hours(10), and 77 hours(11) in water, indicates that hydrolysis, and not volatilization, will be the major fate process for this compound in aquatic environments. According to a classification scheme(5), an estimated BCF of 3(SRC), from an estimated log Kow of -0.7(6) and a regression-derived equation(7), suggests the potential for bioconcentration in aquatic organisms is low(SRC). Biodegradation data were not available(SRC, 2007).ATMOSPHERIC FATE: According to a model of gas/particle partitioning of semivolatile organic compounds in the atmosphere(1), methyl methanesulfonate, which has an estimated vapor pressure of 0.3 mm Hg at 25 °C(SRC), determined from a fragment constant method(2), is expected to exist solely as a vapor in the ambient atmosphere. Vapor-phase methyl methanesulfonate is degraded in the atmosphere by reaction with photochemically-produced hydroxyl radicals(SRC)the half-life for this reaction in air is estimated to be 70 days(SRC), calculated from its rate constant of 2.3X10-13 cu cm/molecule-sec at 25 °C(SRC) that was derived using a structure estimation method(3). Methyl methanesulfonate does not contain chromophores that absorb at wavelengths >290 nm(4) and therefore is not expected to be susceptible to direct photolysis by sunlight(SRC).
有害作用Occupational hepatotoxin - Secondary hepatotoxins: the potential for toxic effect in the occupational setting is based on cases of poisoning by human ingestion or animal experimentation.
Effluent ConcentrationsTire leachate water, obtained from tire cores exposed to water for 40 days, did not contain methyl methanesulfonate at concentrations greater than the detection limit of 1.0 ug/L(1). Methyl methanesulfonate was not detected over a two-year period, from 1989-1991, from the incinerator stack gas(both particulate and vapor), the turbine stack gas(both particulate and vapor), offgas from wastewater treatment, or the sweet syngas of a Louisiana gasification plantGC/MS was used for detection(2).
Medical SurveillancePRECAUTIONS FOR "CARCINOGENS": Whenever medical surveillance is indicated, in particular when exposure to a carcinogen has occurred, ad hoc decisions should be taken concerning ... /cytogenetic and/or other/ tests that might become useful or mandatory. /Chemical Carcinogens/
Soil Adsorption/MobilityUsing a structure estimation method based on molecular connectivity indices(1), the Koc of methyl methanesulfonate can be estimated to be 12(SRC). According to a classification scheme(2), this estimated Koc value suggests that methyl methanesulfonate is expected to have very high mobility in soil.
TSCA Test SubmissionsThe effects of acute oral exposure to methyl methanesulfonate (MMS) (0, 100, 200, 300 or 400 mg/kg) by gavage in male Wistar rats (20 in control group (water), 5/treated group, number of treated groups not reported) were determined. The rats were necropsied on the 11th day following dosing. There were differences between treated and control groups in the following: reduced body weight gain, increased lethargy and a single death in each group (300 and 400 mg/kg dose-levels). There were differences between treated and control animals at the highest dose level in the following: increase in sperm head count (at 300 mg/kg), and a highly significant increase in the percentage of abnormal sperm heads (all levels) including straight heads (i.e. no hook), excessive curvature, folded, coiled, thin or amorphous heads (and excessively elongated/tapering heads, at high-dose level only). There were no significant differences between treated and control animals in the following: histopathology of the testes or testes weights.The effects of acute exposure to methyl methanesulfonate (MMS) (0 or 100 mg/kg) by intraperitoneal injection (in water) in male Wistar rats (6/group) were determined. The rats were necropsied on the 11th day following dosing. There were differences between treated and control groups in the following: markedly reduced body weight gain, increased lethargy, reduced testes weights, and a highly significant increase in sperm head count. There was a "qualitative observation" of an increase in sperm head abnormalities.
Human Toxicity Excerpts/HUMAN EXPOSURE STUDIES/ Therapeutic application of total doses of between 2.8-800 mg/kg body weight over a period of up to 350 days to 13 cancer patients led to significant gastrointestinal and hepatic toxic effects./EPIDEMIOLOGY STUDIES/ Sixty-one xeroderma pigmentosum patients living in the Federal Republic of Germany were investigated. Clinical symptoms were correlated with DNA repair parameters measured in fibroblasts grown from skin biopsies. Classification according to the international complementation groups revealed that of the 61 patients 3 belonged to group A, 26 to group C, 16 to group D, 3 to group E, and 2 to group F11 were of the xeroderma pigmentosum variant type. A striking clinical aspect was the frequency of histogenetically different skin tumors varying from one xeroderma pigmentosum complementation group to the other: squamous and basal cell carinomas predominated in xeroderma pigmentosum group Clentigo maligna melanomas were most frequent in group Dbasal cell carcinomas occurred preferentially in group E and xeroderma pigmentosum variants. Three DNA repair parameters were determined for 46 fibroblast strains: colony forming abilityDNA repair synthesisand DNA incising capacity. Dose response experiments with up to 13 dose levels were performed throughout to achieve sufficient experimental accuracy. DNA damaging treatments included UV light, the UV like carcinogen N-acetoxy-2-acetylaminofluorene, and the alkylating carcinogens methyl methanesulfonate and N-methyl-N-nitrosourea. Comparison of clinical signs and repair data was made on the basis of colony forming ability, DNA repair synthesis, and DNA incising capacity values of both individual cell strains and weighted means of xeroderma pigmentosum complementation groups. Despite considerable clinical and biochemical heterogeneity within complementation groups distinctive features emerged. In general, colony forming ability, DNA repair synthesis, and DNA incising capacity values of all xeroderma pigmentosum strains investigated, including xeroderma pigmentosum variants, were found to be reduced upon treatment with UV light or N-acetoxy-2-acetylaminofluorene. After treatment with UV light or N-acetoxy-2-acetylaminofluorene, cell strains in which DNA incising capacity was reduced also showed a similar reduction in both colony forming ability and DNA repair synthesis. Consequently, the weighted mean colony forming ability, DNA repair synthesis, and DNA incising capacity values of complementation groups and xeroderma pigmentosum variants correlated with each other. Furthermore, the onset of both early dermatological symptoms of xeroderma pigmentosum and tumor growth correlated with the extent of DNA repair defects. Of 45 xeroderma pigmentosum fibroblast strains checked for colony forming ability after treatment with methyl methanesulfonate only 3 cell strains from group D were found to be more sensitive than normal controls, suggesting that overall repair in xeroderma pigmentosum strains was equal to that in controls. Weighted means of DNA repair synthesis of xeroderma pigmentosum complementation groups, however, showed reductions hinting at impaired excision of distinct alkylated bases. This held true for complementation groups, D, A, E, and F. Upon treatment with N-methyl-N-nitrosourea, the weighted mean DNA repair synthesis values of the complementation groups did not differ significantly from that of controls, ... However, the weighted mean colony forming ability value of complementation group D was significantly reduced, .../ALTERNATIVE and IN VITRO TESTS/ Nine human tumor cell lines derived from both epithelial and mesenchymal tumors exhibited either an anchorage independent growth non-tumorigenic phenotype or an anchorage independent tumorigenic phenotype. Transformed epithelial cell lines with the non-tumorigenic phenotype could be converted to a progressively growing tumor phenotype following treatment with either methylmethane sulfonate or N-methyl-N'-nitro-N-nitrosoguanidine. In contrast, sarcoma derived cell lines with a non-tumorigenic phenotype could be converted to a progressively growing tumor phenotype only with N-methyl-N'-nitro-N-nitrosoguanidine. SV40 immortalized HET-1A non-tumorigenic phenotype cells could be converted to a progressively growing tumorigenic phenotype, infrequently, when treated with N-methyl-N'-nitro-N-nitrosoguanidine, but not methylmethane sulfonate. Progressively growing tumors produced by either methylmethane sulfonate or N- methyl-N'-nitro-N-nitrosoguanidine treated non-tumorigenic phenotypes exhibited metastatic potential in nude mice. Chemically treated HET-1A cells acquired the abilty to produce tumor in mice but the tumor did not exhibit metastatic potential. In contrast, populations of tumorigenic cells were not rendered more biologically aggressive after treatment with either methylmethane sulfonate or N-methyl-N'-nitro-N-nitrosoguanidineie, the latency period for tumor development was not accelerated and the tumors did not exhibit metastatic potential. .../ALTERNATIVE and IN VITRO TESTS/ Squamous epithelial carcinoma is a predominant form of skin cancer in man and, in theory, human epidermal keratinocytes present an appropriate target cell to employ as an in vitro system to study epidermal carcinogenesis. Keratinocytes were serially cultivated from adult human skin samples and maintained in culture for at least 3 passages. Tertiary cultures, isolated from 3 separate individuals, were exposed to the direct acting experimental carcinogen, methyl methanesulfonate. DNA repair was assessed by a quantitative autoradiographic technique. Methyl methanesulfonate elicited a dose related increase in unscheduled DNA synthesis in cultures prepared from each individual. Inter-individual variation in the response was observed.For more Human Toxicity Excerpts (Complete) data for METHYL METHANESULFONATE (8 total), please visit the HSDB record page.
Artificial Pollution SourcesMethyl methanesulfonate's former production and use as a research chemical(1) may have resulted in its limited release to the environment through various waste streams(SRC).
Carcinogen ClassificationMethyl methanesulfonateGroup 2A: Probably carcinogenic to humansVolume 7: (1974) Some Anti-thyroid and Related Substances, Nitrofurans and Industrial Chemicals19991998NB Overall evaluation upgraded to Group 2A with supporting evidence from other relevant data
Environmental BioconcentrationAn estimated BCF of 3 was calculated in fish for methyl methanesulfonate(SRC), using an estimated log Kow of -0.7(1) and a regression-derived equation(2). According to a classification scheme(3), this BCF suggests the potential for bioconcentration in aquatic organisms is low(SRC).
F21
水環境危害等級（WGK）3
TSCA 収載状態TSCA listed
RTECS 番号PB2625000
輸送番号（UN）UN 2810 6.1/PG 3
毒性LD50 orally in Rabbit: 225 mg/kg
税関コード2905 19 00
危険文句（R）45-22-36/37/38-68
安衛法変異原性物質
消防法危-4-3-III
危険表示T
安全措置（S）53-26-45-36/37/39
危険等級6.1(b)Acute Tox. 3 OralAquatic Chronic 2Carc. 2Eye Irrit. 2Muta. 1BRepr. 2Skin Irrit. 2Skin Sens. 1STOT RE 2 Inhalation
包装等級III
貯蔵分類6.1C - Combustible acute toxic Cat.3toxic compounds or compounds which causing chronic effects
有害物質データ66-27-3(Hazardous Substances Data)