卵胞刺激ホルモン

解説生殖腺刺激ホルモンの一。脳下垂体前葉から分泌されるホルモンで、卵巣の卵胞を成熟させ、エストロゲンの分泌を促進させる。FSH（follicle-stimulating hormone）。小学館 デジタル大辞泉について 情報 | 凡例

説明Gonal-F was launched in Austria, Germany, Denmark, Ireland, Italy, Norway, Sweden, Switzerland, France and the UK for the treatment of infertility. The recombinant follicule stimulating hormone (recFSH) was produced by introducing the genes for the α- and β-FSH subunits into the genome of Chinese hamster ovary cells. The isolated recFSH was a glycosylated bioactive dimer that appeared to be identical to urinary human FSH. Biochemically, the recFSH was equal to or slightly better to the uroFSH. The ultra-high purity of recFSH makes it less prone to side effects from contaminants in uroFSH and can be injectable.

天然物の起源ヒト下垂体前葉，α 鎖：25～116, β鎖：19～129

使用Follicle stimulating hormone (FSH) from human pituitary has been used for-in vitro luteinization of Macaque granulosa cellsluteal cell proliferation assayculturing of preantral follicle cells obtained from micein vitro maturation of immature cumulus-oocyte complexes (COCs) obtained from mice andculture of RAW264.7 cell line

一般的な説明The α chain of FSHhas 89 amino acids and the β chain has 115 amino acids. The α chain is not active. FSH belongs to glycoprotein hormone family. It is secreted by the gonadotropic cells of pituitary gland.

純化方法FSH is purified by Sephadex G100 gel filtration followed by carboxymethyl-cellulose with NH4OAc pH 5.5. The latter separates luteinising hormone from FSH. Its solubility in H2O is 0.5%. It has an isoelectric point of 4.5. A solution of 1mg in saline (100mL) can be kept at 60o for 0.5hour. Activity is retained in a solution at pH 7-8 for 0.5hour at 75o. The activity of a 50% aqueous EtOH solution is destroyed at 60o in 15minutes. [Bloomfield et al. Biochim Biophys Acta 533 371 1978, Hartree Biochem J 100 754 1966, Pierce & Parsons Ann Rev Biochem 50 465 1981.]