1-フェニル-2-チオ尿素

Signs and Symptoms of Phenylthiourea Exposure: Acute exposure to phenylthiourea may result in vomiting, difficult breathing, noisy breathing, cyanosis, and low body temperature. Antithyroid activity and hyperglycemia may be observed after chronic sublethal exposure.

Suitable extinguishing media: Use water spray, alcohol-resistant foam, dry chemical or carbon dioxide.

Advice for firefighters: Wear self-contained breathing apparatus for firefighting if necessary.

Rooms used only for storage should be soundly constructed and fitted with secure locks. Floors should be kept clear and the pesticides clearly identified. /Pesticides/

Conditions for safe storage, including any incompatibilities: Keep container tightly closed in a dry and well-ventilated place.

Spillages often occur in storage and repacking rooms, and they must be cleaned up with care. /Pesticides/

Accidental Release Measures. Personal precautions, protective equipment and emergency procedures: Wear respiratory protection. Avoid dust formation. Avoid breathing vapours, mist or gas. Ensure adequate ventilation. Evacuate personnel to safe areas. Avoid breathing dust. Environmental precautions: Prevent further leakage or spillage if safe to do so. Do not let product enter drains. Methods and materials for containment and cleaning up: Pick up and arrange disposal without creating dust. Sweep up and shovel. Keep in suitable, closed containers for disposal.

Generators of waste (equal to or greater than 100 kg/mo) containing this contaminant, EPA hazardous waste number P093, must conform with USEPA regulations in storage, transportation, treatment and disposal of waste.

A potential candidate for rotary kiln incineration at a temperature range of 820 to 1600 °C and residence times of seconds for liquids and gases, and hours for solids. A potential candidate for fluidized bed incineration at a temperature range of 450 to 980 °C and residence times of seconds for liquidsand gases, and longer for solids.

Waste treatment methods. Product: Offer surplus and non-recyclable solutions to a licensed disposal company. Contact a licensed professional waste disposal service to dispose of this material. Dissolve or mix the material with a combustible solvent and burn in a chemical incinerator equipped with an afterburner and scrubber. Contaminated packaging: Dispose of as unused product.

... Eating, drinking and smoking should be prohibited /when pesticides are being handled./

Precautions for safe handling: Avoid contact with skin and eyes. Avoid formation of dust and aerosols. Provide appropriate exhaust ventilation at places where dust is formed.Normal measures for preventive fire protection.

Appropriate engineering controls: Avoid contact with skin, eyes and clothing. Wash hands before breaks and immediately after handling the product.

Gloves must be inspected prior to use. Use proper glove removal technique (without touching glove's outer surface) to avoid skin contact with this product. Dispose of contaminated gloves after use in accordance with applicable laws and good laboratory practices. Wash and dry hands.

SRP: Local exhaust ventilation should be applied wherever there is an incidence of point source emissions or dispersion of regulated contaminants in the work area. Ventilation control of the contaminant as close to its point of generation is both the most economical and safest method to minimize personnel exposure to airborne contaminants. Ensure that the local ventilation moves the contaminant away from the worker.

0.0090 [mg/m3]

0.099 [mg/m3]

0.59 [mg/m3]

For emergency situations, wear a positive pressure, pressure-demand, full facepiece self-contained breathing apparatus (SCBA) or pressure- demand supplied air respirator with escape SCBA and a fully-encapsulating, chemical resistant suit. (EPA, 1998)

Eye/face protection: Face shield and safety glasses Use equipment for eye protection tested and approved under appropriate government standards such as NIOSH (US) or EN 166(EU).

Skin protection: Handle with gloves.

Body Protection: Complete suit protecting against chemicals, The type of protective equipment must be selected according to the concentration and amount of the dangerous substance at the specific workplace.

Respiratory protection: Where risk assessment shows air-purifying respirators are appropriate use a full-face particle respirator type N100 (US) or type P3 (EN 143) respirator cartridges as a backup to engineering controls. If the respirator is the sole means of protection, use a full-face supplied air respirator. Use respirators and components tested and approved under appropriate government standards such as NIOSH (US) or CEN (EU).

The present study examined the combined effect of dopamine and 1-methyl-4-phenylpyridinium (MPP(+)) on the membrane permeability in isolated brain mitochondria and on cell viability in PC12 cells. MPP(+) increased effect of dopamine against the swelling, membrane potential, and Ca(2+) transport in isolated mitochondria, which was not inhibited by the addition of antioxidant enzymes (SOD and catalase). Dopamine or MPP(+) caused the decrease in transmembrane potential, increase in reactive oxygen species, depletion of GSH, and cell death in PC12 cells. Antioxidant enzymes reduced each effect of dopamine and MPP(+) against PC12 cells. Co-addition of dopamine and MPP(+) caused the decrease in the transmembrane potential and increase in the formation of reactive oxygen species in PC12 cells, in which they showed an additive effect. Dopamine plus MPP(+)-induced the depletion of GSH and cell death in PC12 cells were not decreased by the addition of antioxidant enzymes, rutin, diethylstilbestrol, and ascorbate. Melanin caused a cell viability loss in PC12 cells. The N-acetylcysteine, N-phenylthiourea, and 5-hydroxyindole decreased the cell death and the formation of dopamine quinone and melanin induced by co-addition of dopamine and MPP(+), whereas deprenyl and chlorgyline did not show an inhibitory effect. The results suggest that co-addition of dopamine and MPP(+) shows an enhancing effect on the change in mitochondrial membrane permeability and cell death, which may be accomplished by toxic quinone and melanin derived from the MPP(+)-stimulated dopamine oxidation.

Pretreatment of animals with 1-methyl-1-phenylthiourea prevents desulfuration & reduces toxicity of PTU.

Cysteine & reduced glutathione partially reduced the liver glycogen-depleting effect of a toxic phenylthiourea dose in rats.

In vitro binding of (14)Carbon thiourea to rat lung protein was antagonized by the presence of phenylthiourea.

For more Interactions (Complete) data for 1-PHENYL-2-THIOUREA (7 total), please visit the HSDB record page.

TERRESTRIAL FATE: Based on a classification scheme(1), an estimated Koc value of 100(SRC), determined from a structure estimation method(2), indicates that 1-phenyl-2-thiourea is expected to have high mobility in soil(SRC). The estimated pKa of 1-phenyl-2-thiourea is 9.62(3), indicating that this compound will exist partially in the anion form in the environment and anions generally do not adsorb more strongly to soils containing organic carbon and clay than their neutral counterparts(4). Volatilization of the anion form of 1-phenyl-2-thiourea from moist soil surfaces is not expected because anions do not volatilize(SRC). Volatilization of neutral 1-phenyl-2-thiourea from moist soil surfaces is not expected to be an important fate process(SRC) given an estimated Henry's Law constant of 8.7X10-8 atm-cu m/mole(SRC), using a fragment constant estimation method(2). 1-Phenyl-2-thiourea is not expected to volatilize from dry soil surfaces(SRC) based upon an estimated vapor pressure of 2.8X10-4 mm Hg at 25 °C(SRC), determined from a fragment constant method(2). Biodegradation data in soil were not available(SRC, 2014).

AQUATIC FATE: Based on a classification scheme(1), an estimated Koc value of 100(SRC), determined from a structure estimation method(2), indicates that 1-phenyl-2-thiourea is expected to adsorb to suspended solids and sediment(SRC). An estimated pKa of 9.62(3) indicates 1-phenyl-2-thiourea will exist partially in the anion form at pH values of 5 to 9 and, therefore, volatilization of the anion form from water surfaces is not expected to be an important fate process(SRC). Volatilization of the neutral form from water surfaces is not expected(4) based upon an estimated Henry's Law constant of 8.7X10-8 atm-cu m/mole(SRC), developed using a fragment constant estimation method(2). 1-Phenyl-2-thiourea is not expected to undergo hydrolysis in the environment due to the lack of functional groups that hydrolyze under environmental conditions(4). According to a classification scheme(5), an estimated BCF of 3(SRC), from its log Kow of 0.71(6) and a regression-derived equation(2), suggests the potential for bioconcentration in aquatic organisms is low(SRC). Biodegradation data in water were not available(SRC, 2014).

ATMOSPHERIC FATE: According to a model of gas/particle partitioning of semivolatile organic compounds in the atmosphere(1), 1-phenyl-2-thiourea, which has an estimated vapor pressure of 2.8X10-4 mm Hg at 25 °C(SRC), determined from a fragment constant method(2), is expected to exist solely as a vapor in the ambient atmosphere. Vapor-phase 1-phenyl-2-thiourea is degraded in the atmosphere by reaction with photochemically-produced hydroxyl radicals(SRC); the half-life for this reaction in air is estimated to be 5 hours(SRC), calculated from its rate constant of 6.3X10-11 cu cm/molecule-sec at 25 °C(SRC) that was derived using a structure estimation method(2). 1-Phenyl-2-thiourea contains chromophores that absorb at wavelengths >290 nm(3) and, therefore, may be susceptible to direct photolysis by sunlight(SRC).

IDENTIFICATION AND USE: 1-Phenyl-2-thiourea is a bitter or tasteless (depending on the individual's genetic background) needle- or prism-like solid material used in medical genetics studies, as a repellent for rats, rabbits, and weasels, and in the production of rodenticides. HUMAN EXPOSURE AND TOXICITY: 1-Phenyl-2-thiourea can be absorbed by ingestion and parenterally; it may be absorbed and cause systemic toxicity following inhalation or dermal exposure. The bitter taste perception (associated with the ability or inability to taste 1-phenyl-2-thiourea) is mediated by the tas2r38 gene. Some studies have found higher proportions of nontasters among those with a family history of alcoholism than controls, whereas others have not. ANIMAL STUDIES: Vomiting, noisy or difficult breathing, cyanosis, and hypothermia may occur. Pleural effusions and pulmonary edema have been seen in exposed experimental animals, and this compound destroys cytochrome P450 in vivo. Hypoglycemia lasting for up to 5 hours was seen in rats injected with 1-phenyl-2-thiourea. In mice it was nearly as effective as a strong dose of licl in reducing sucrose drinking. 1-Phenyl-2-thiourea produced a concn-dependent inhibition of rat lung acetylcholinesterase activity in vitro. Phenylthiourea caused mutagenicity in the Salmonella typhimurium assay without S9 activation, but was negative in the salmonella/microsome preincubation assay. At the standard concentration of 0.003% (200 uM), 1-phenyl-2-thiourea inhibits melanogenesis and reportedly has minimal other effects on zebrafish embryogenesis. The administration of 0.003% 1-phenyl-2-thiourea altered retinoic acid and insulin-like growth factor regulation of neural crest and mesodermal components of craniofacial development. 1-Phenyl-2-thiourea also decreased retinoic acid-induced teratogenic effects on pharyngeal arch and jaw cartilage despite morphologically normal appearing 1-phenyl-2-thiourea -treated controls. 1-Phenyl-2-thiourea inhibited neural crest development at higher concentrations (0.03%) and had the greatest inhibitory effect when added prior to 22 hours post fertilization (hpf). Addition of 0.003% 1-phenyl-2-thiourea between 4 and 20 hpf decreased thyroxine (T4) in thyroid follicles in the nasopharynx of 96 hpf embryos. 1-Phenyl-2-thiourea is an experimental teratogen in mice. In NTP 78 weeks feeding study 1-phenyl-2-thiourea was not carcinogenic to rats (120 and 60 ppm) and mice (300 and 150 ppm) in both males or females.

The following link will take the user to the National Toxicology Program (NTP) Test Agent Search Results page, which tabulates all of the "Standard Toxicology & Carcinogenesis Studies", "Developmental Studies", and "Genetic Toxicity Studies" performed with this chemical. Clicking on the "Testing Status" link will take the user to the status (i.e., in review, in progress, in preparation, on test, completed, etc.) and results of all the studies that the NTP has done on this chemical.[Available from, as of October 20, 2014: http://ntp-apps.niehs.nih.gov/ntp_tox/index.cfm?fuseaction=ntpsearch.searchresults&searchterm=103-85-5]

Using a structure estimation method based on molecular connectivity indices(1), the Koc of 1-phenyl-2-thiourea can be estimated to be 100(SRC). According to a classification scheme(2), this estimated Koc value suggests that 1-phenyl-2-thiourea is expected to have high mobility in soil. The estimated pKa of 1-phenyl-2-thiourea is 9.62(3), indicating that this compound will exist partially in anion form in the environment and anions generally do not adsorb more strongly to soils containing organic carbon and clay than their neutral counterparts(4).

/SIGNS AND SYMPTOMS/ Information profiles were presented for thiourea and the following thiourea compounds considered important in the industrial community: 1-allyl-3-(2-hydroxyethyl)-2-thiourea (105817), allylthiourea (109579), N,N'-bis(hydroxymethyl)thiourea (3084251), N,N'-bis(methoxymethyl)thiourea (51872265), 1-butyl-3-ethyl-2-thiourea (32900064), 1,3-dibutyl-2-thiourea (109466), 1,3-diisopropyl-2-thiourea (2986176), 1,3-dimethyl-2-thiourea (534134), N,N'-di-o-tolylthiourea (137973), 3-ethyl-1,1-dimethyl-2-thiourea (15361861), ethylthiourea (625536), (hydroxymethyl)thiourea (1000835), (2-methylphenyl)thiourea (614788), thiourea (62566), 1,3-diethyl-2-thiourea (105555), 1,3-diphenyl-2-thiourea (102089), ethylenethiourea (96457), 1-naphthylthiourea (86884), 1-phenyl-2-thiourea (103855), 1,1,3,3-tetramethylthiourea (2782914) and 1,1,3-trimethyl-2-thiourea (2489772)... Positive mutagenic effects have been elicited in various assays with several of these compounds. The use of thiourea, diethylthiourea and tetramethylthiourea as antithyroid drugs indicated these compounds have antithyroidal effects in humans. Frequently observed side effects from the use of these medications have included headache, anxiety, fever, skin rash, and gastrointestinal disturbances. /thioureas/

/SIGNS AND SYMPTOMS/ In the manufacture of rubber, irritant contact dermatitis may occur from a variety of acids, alkalis, detergents, and solvents used in the process. Allergic contact dermatitis occurs not infrequently and is almost always due to an organic accelerator or antioxidant. While the list of potential sensitizing accelerators and antioxidants is enormous, common allergens include ... thioureas. /Thioureas/

/EPIDEMIOLOGY STUDIES/ The bitter taste perception (associated with the ability or inability to taste phenylthiocarbamide) is mediated by the TAS2R38 gene. Most of the variation in this gene is explained by three common amino-acid polymorphisms at positions 49 (encoding proline or alanine), 262 (alanine or valine) and 296 (valine or isoleucine) that determine two common isoforms: proline-alanine-valine (PAV) and alanine-valine-isoleucine (AVI). PAV is the major taster haplotype (heterozygote and homozygote) and AVI is the major non-taster haplotype (homozygote). Amino acid 49 has the major effect on the distinction between tasters and non-tasters of all three variants. The sense of bitter taste protects us from ingesting toxic substances, present in some vegetables, that can affect the thyroid when ingested in large quantities. Balancing selection has been used to explain the current high non-taster frequency, by maintaining divergent TAS2R38 alleles in humans. /Researchers/ have amplified and sequenced the TAS2R38 amino acid 49 in the virtually uncontaminated Neanderthal sample of El Sidron 1253 and have determined that it was heterozygous. Thus, this Neanderthal was a taster individual, although probably slightly less than a PAV homozygote. This indicates that variation in bitter taste perception pre-dates the divergence of the lineages leading to Neanderthals and modern humans.

1-Phenyl-2-thiourea's production and use in medical genetics(1) and as an ingredient in silver polishes may result in its release to the environment through various waste streams(SRC). It's limited use as an inert ingredient in residential pesticides(2) will result in its direct release to the environment(SRC).

1-Phenyl-2-thiourea

TR-148: Bioassay of 1-Phenyl-2-thiourea for Possible Carcinogenicity (CASRN 103-85-5) (1978 )

06/25/78

No Evidence

No Evidence

No Evidence

An estimated BCF of 3 was calculated in fish for 1-phenyl-2-thiourea(SRC), using a log Kow of 0.71(1) and a regression-derived equation(2). According to a classification scheme(3), this BCF suggests the potential for bioconcentration in aquatic organisms is low(SRC).

An estimated pKa of 9.62(1) indicates 1-phenyl-2-thiourea will exist partially in the anion form at pH values of 5 to 9 and, therefore, volatilization of the ionic form from water and moist surfaces is not expected to be an important fate process(SRC). The Henry's Law constant for 1-phenyl-2-thiourea is estimated as 8.7X10-8 atm-cu m/mole(SRC) using a fragment constant estimation method(2). This Henry's Law constant indicates that neutral 1-phenyl-2-thiourea is expected to be essentially nonvolatile from water and moist soil surfaces(3). 1-Phenyl-2-thiourea is not expected to volatilize from dry soil surfaces(SRC) based upon an estimated vapor pressure of 2.8X10-4 mm Hg(SRC), determined from a fragment constant method(2).

/LABORATORY ANIMALS: Acute Exposure/ Previous work has demonstrated that SWR/J (SW) mice avoid phenylthiocarbamide (PTC) to a greater degree than C3HeB/FeJ mice in 48 hr, two-bottle preference tests given in ascending series. The authors hypothesized, based also on previous work, that SW mice might form a conditioned taste aversion over time due to the toxic properties of PTC. /This study/ directly tested this hypothesis by attempting to condition a taste aversion to sucrose by injections of PTC. In experiment 1, PTC was nearly as effective as a strong dose of LiCl in reducing sucrose drinking. In experiment 2, the sucrose aversions were parametrically modified by both sucrose concentration and PTC dose, a hallmark of conditioned taste aversion. /This study/ concludes that PTC can cause a conditioned taste aversion and discuss the importance of considering toxic effects of aversive tastants when analyzing behavioral strain differences.

/LABORATORY ANIMALS: Acute Exposure/ POISONED RATS EXHIBITED PLEURAL EFFUSIONS & PULMONARY EDEMA. ... MICE GIVEN MASSIVE DOSES OF PTU (100 MG/KG) IP SURVIVED AT LEAST 8 HR ALTHOUGH ALL HAD DIED BY 22 HR. SIMILARLY, RATS GIVEN 25 MG/KG IP SURVIVED MANY HR.

/LABORATORY ANIMALS: Developmental or Reproductive Toxicity/ Teratogenic in mice given 168 mg/kg or higher doses orally on days 1 to 21 of pregnancy.

/LABORATORY ANIMALS: Developmental or Reproductive Toxicity/ 1-Phenyl 2-thiourea (PTU) is a tyrosinase inhibitor commonly used to block pigmentation and aid visualization of zebrafish development. At the standard concentration of 0.003% (200 uM), PTU inhibits melanogenesis and reportedly has minimal other effects on zebrafish embryogenesis. /The study/ found that 0.003% PTU altered retinoic acid and insulin-like growth factor (IGF) regulation of neural crest and mesodermal components of craniofacial development. Reduction of retinoic acid synthesis by the pan-aldehyde dehydrogenase inhibitor diethylbenzaldehyde, only when combined with 0.003% PTU, resulted in extraocular muscle disorganization. PTU also decreased retinoic acid-induced teratogenic effects on pharyngeal arch and jaw cartilage despite morphologically normal appearing PTU-treated controls. Furthermore, 0.003% PTU in combination with inhibition of IGF signaling through either morpholino knockdown or pharmacologic inhibition of tyrosine kinase receptor phosphorylation, disrupted jaw development and extraocular muscle organization. PTU in and of itself inhibited neural crest development at higher concentrations (0.03%) and had the greatest inhibitory effect when added prior to 22 hours post fertilization (hpf). Addition of 0.003% PTU between 4 and 20 hpf decreased thyroxine (T4) in thyroid follicles in the nasopharynx of 96 hpf embryos. Treatment with exogenous triiodothyronine (T3) and T4 improved, but did not completely rescue, PTU-induced neural crest defects. Thus, PTU should be used with caution when studying zebrafish embryogenesis as it alters the threshold of different signaling pathways important during craniofacial development. The effects of PTU on neural crest development are partially caused by thyroid hormone signaling.

The rate constant for the vapor-phase reaction of 1-phenyl-2-thiourea with photochemically-produced hydroxyl radicals has been estimated as 6.4X10-11 cu cm/molecule-sec at 25 °C(SRC) using a structure estimation method(1). This corresponds to an atmospheric half-life of about 5 hours at an atmospheric concentration of 5X10+5 hydroxyl radicals per cu cm(1). 1-Phenyl-2-thiourea is not expected to undergo hydrolysis in the environment due to the lack of functional groups that hydrolyze under environmental conditions(2). 1-Phenyl-2-thiourea contains chromophores that absorb at wavelengths >290 nm(2) and, therefore, may be susceptible to direct photolysis by sunlight(SRC).

P093; As stipulated in 40 CFR 261.33, when phenylthiourea, as a commercial chemical product or manufacturing chemical intermediate or an off-specification commercial chemical product or a manufacturing chemical intermediate, becomes a waste, it must be managed according to federal and/or state hazardous waste regulations. Also defined as a hazardous waste is any container or inner liner used to hold this waste or any residue, contaminated soil, water, or other debris resulting from the cleanup of a spill, into water or on dry land, of this waste. Generators of small quantities of this waste may qualify for partial exclusion from hazardous waste regulations (40 CFR 261.5(e)).

Persons in charge of vessels or facilities are required to notify the National Response Center (NRC) immediately, when there is a release of this designated hazardous substance, in an amount equal to or greater than its reportable quantity of 100 lb or 45.4 kg. The toll free number of the NRC is (800) 424-8802. The rule for determining when notification is required is stated in 40 CFR 302.4 (section IV. D.3.b).

Releases of CERCLA hazardous substances are subject to the release reporting requirement of CERCLA section 103, codified at 40 CFR part 302, in addition to the requirements of 40 CFR part 355. Phenylthiourea is an extremely hazardous substance (EHS) subject to reporting requirements when stored in amounts in excess of its threshold planning quantity (TPQ) of 100 or 10,000 lbs. Extremely hazardous substances that are solids are subject to either of two threshold planning quantities ... The lower quantity applies only if the solid exists in powdered form and has a particle size less than 100 microns; or is handled in solution or in molten form; or meets the criteria for a National Fire Protection Association (NFPA) rating of 2, 3 or 4 for reactivity. If the solid does not meet any of these criteria, it is subject to the upper ... threshold planning quantity ... .

Used to make rodenticides, as repellant for rats, rabbits, and weasels, and in medical genetics; [HSDB]

In medical genetics.

Ingredient in silver polishes and residential pesticides

(1976) 1.03X10+6 GRAMS (PRINCPL CUSTMS DISTS)

(1978) 1.00X10+6 GRAMS (PRINCPL CUSTMS DISTS)

Thiourea, N-phenyl-: ACTIVE

Phenylthiourea is a repellent for rats, rabbits, and weasels. Effective concn are 0.012-0.014%, & can be lowered to 0.002-0.003% by addition of 5% sucrose.