基本情報
- CAS
92-32-0- 標準SMILES
CN(C)c1ccc2cc3ccc(=[N+](C)C)cc-3oc2c1.[Cl-]- InChIKey
INCIMLINXXICKS-UHFFFAOYSA-M- 分子式
- C17H19N2O.Cl
- 平均分子量
- 302.8 g/mol
- 単同位体質量
- 302.1185909
概要
Pyronin Y is an organic chloride salt having 6-(dimethylamino)-N,N-dimethyl-3H-xanthen-3-iminium as the cation. Used with methyl green to selectively demonstrate RNA (red) in contrast to DNA (green) with the Unna-Pappenheim method. It has a role as a histological dye. It is an iminium salt and an organic chloride salt. It contains a pyronin Y cation.
名称と識別子
- 優先名称
- Pyronine
- IUPAC
- [6-(dimethylamino)xanthen-3-ylidene]-dimethylazanium chloride
- 現地名
- ピロニン
- 英語名
- PYRONIN Y
- 分子式
- C17H19ClN2O
- 分子量
- 302.8
名称と別名
Pyronin Y92-32-0Pyronine YPyroninePyronin GPyronin YellowPyronine GTetramethyl pyronin3,6-Bis(dimethylamino)xanthylium chlorideMethyl pyroninXanthylium, 3,6-bis(dimethylamino)-, chloridePyronine ZH
名称をさらに表示
ピロニンイエローピロニンYピロニンG3,6-ビス(ジメチルアミノ)キサンチリウム·クロリドピロニンGSシュルツNo.853ピロニンGYピロニン YピロニンY, 高純度ピロニンPyonin GPyonin YCI 45005PYRONIN J
C.I. 45005tetramethyldiaminoxanthylium chlorideSchultz No. 853NSC-10454W659G165T1CHEBI:8682DTXSID8059051CI-45005
データベース識別子
- CAS
92-32-0- PubChem CID
- 7085
- DTXSID
DTXSID8059051- ChEMBL
CHEMBL1486690
物性
構造計算による物性
- 極性表面積
- 15.5 Ų
- 水素結合供与体数
- 0
- 水素結合受容体数
- 3
- 回転可能結合数
- 1
- 重原子数
- 21
- 形式電荷
- 0
- 複雑度
- 480
実験および物理化学的性質
Chemical ClassesDyes -> Acridine Dyes
Physical Description
Liquid
Lustrous green solid; [Merck Index] Iridescent green crystals; [Amresco MSDS]
物理化学的性質
- 融点
- 250 · 250-260 °C(lit.)
- 貯蔵温度
- room temp
- 溶解性
- 水に溶けます。エタノール、エチレングリコール、メチルセロソルブにやや溶けにくい
- 外見
- 粉体・固形
- 色
- ピンク
- 水溶解度
- 水に溶かす。メチル セロソルブ (9 mg/mL) およびエタノール (3 mg/mL) にも可溶です。
- Merck
- 13 · 13,8097
- BRN
- 3795789 · 3795789
- 安定性
- 安定。強力な酸化剤とは相容れない。
- 主な用途
- 診断アッセイの製造血液学組織学
- InChI
- 1S/C17H19N2O.ClH/c1-18(2)14-7-5-12-9-13-6-8-15(19(3)4)11-17(13)20-16(12)10-14;/h5-11H,1-4H3;1H/q+1;/p-1
- InChIKey
- INCIMLINXXICKS-UHFFFAOYSA-M
- SMILES
- [Cl-].CN(C)c1ccc2C=C3C=C\C(C=C3Oc2c1)=[N+](/C)C
- CAS データベース
- 92 · 92-32-0
安全と法規制
安全情報は参考情報です。供給元の安全データシート（SDS）、適用法令、専門家による評価に代わるものではありません。
GHS分類
GHS Classification
Warning
H341 (100%): Suspected of causing genetic defects [Warning Germ cell mutagenicity]
P203, P280, P318, P405, and P501 (click each P-code to see the statement)
The GHS information provided by 1 company from 1 notification to the ECHA C&L Inventory.
危険有害性情報
Regulatory Information
Chemical: Methanaminium, N-[6-(dimethylamino)-3H-xanthen-3-ylidene]-N-methyl-, chloride
Xanthylium, 3,6-bis(dimethylamino)-, chloride (1:1) is listed on the EPA's Chemical Data Reporting (CDR) system. Manufacturers and importers of Xanthylium, 3,6-bis(dimethylamino)-, chloride (1:1) are required to report information about their production and use of this chemical to the EPA under the Toxic Substances Control Act (TSCA). (40 eCFR Part 711)
Xanthylium, 3,6-bis(dimethylamino)-, chloride: Does not have an individual approval but may be used under an appropriate group standard
Hazards SummaryCauses changes in kidneys (acute tubular necrosis) in intravenous lethal-dose studies of mice (LD50 = 179 mg/kg); [RTECS] A skin and strong eye irritant; Targets the kidney and bladder; [Amresco MSDS]
Hazard Classes and CategoriesMuta. 2 (100%)
安全データ
- wgk
- 3
- tsca
- TSCA listed
- rtecs
- BQ1450000
- hs_code
- 32041300
- risk_code
- 68-36/37/38
- hazard_code
- Xn,Xi
- safety_code
- 22-24/25-36-26
- ストレージクラス
- 11 - Combustible Solids
化学と産業
薬理情報
MeSH Pharmacological ClassificationChemicals and substances that impart color including soluble dyes and insoluble pigments. They are used in INKS; PAINTS; and as INDICATORS AND REAGENTS.
用途と製造
UsesUsed as a bacterial and biological stain; [Merck Index]
U.S. Production2022: 11,116 lb
Industry UsesFiller
General Manufacturing Information
All Other Chemical Product and Preparation Manufacturing
Xanthylium, 3,6-bis(dimethylamino)-, chloride (1:1): ACTIVE
応用分野
解説ピロニン，ピロニンG(pyronine G)：C17H19ClN2O(302.80)．N,N-ジメチルアミノフェノールから合成される．緑色の結晶．水溶液は赤色を呈する．λmax 552 nm(エタノール)．【Ⅱ】ピロニンB(pyronine B)：C42H54Cl8Fe2N4O2(1042.24)．鉄との複合体で市販されている．緑色の針状晶．λmax 555 nm(エタノール)．森北出版「化学辞典（第2版）
使用Pyronin Y is a cationic dye that intercalates RNA and is visible as a red band during electrophoresis. It has also been shown to accumulate in mitochondria of viable cells. In conjuction with Methyl Green or Nancy-520, Pyronin Y can be used as a fluorescent differential stain of nucleic acids in paraffin tissue sections.
一般的な説明Pyronin Y (C.I. 45005) is an aminoxanthene dye that is present as a weakly lipophilic cation in neutral aqueous solutions. It is also known as pyronine G.
調製方法
純化方法Commercial material may contain a large quantity of zinc. Purify it by dissolving 1g in 50mL of hot water containing 5g NaEDTA. Cool to 0o, filter, evaporate to dryness and the residue is extracted with EtOH. The solution is evaporated to 5-10mL, filtered, and the dye is precipitated by addition of excess of dry diethyl ether. It is centrifuged, and the crystals are washed with dry ether. The procedure is repeated, then the product is dissolved in CHCl3, filtered and evaporated. The dye is stored in a vacuum. [Beilstein 18 H 596, 18 III/IV 7361, 18/10 V 181.]
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