2H-ベンゾトリアゾール

Fires involving this compound can be controlled with a dry chemical, carbon dioxide or Halon extinguisher. (NTP, 1992)

Use water spray.

Fresh air, rest.

Remove contaminated clothes. Rinse and then wash skin with water and soap.

First rinse with plenty of water for several minutes (remove contact lenses if easily possible), then refer for medical attention.

Rinse mouth. Give one or two glasses of water to drink. Refer for medical attention .

EYES: First check the victim for contact lenses and remove if present. Flush victim's eyes with water or normal saline solution for 20 to 30 minutes while simultaneously calling a hospital or poison control center. Do not put any ointments, oils, or medication in the victim's eyes without specific instructions from a physician. IMMEDIATELY transport the victim after flushing eyes to a hospital even if no symptoms (such as redness or irritation) develop.；SKIN: IMMEDIATELY flood affected skin with water while removing and isolating all contaminated clothing. Gently wash all affected skin areas thoroughly with soap and water. If symptoms such as redness or irritation develop, IMMEDIATELY call a physician and be prepared to transport the victim to a hospital for treatment.；INHALATION: IMMEDIATELY leave the contaminated area; take deep breaths of fresh air. If symptoms (such as wheezing, coughing, shortness of breath, or burning in the mouth, throat, or chest) develop, call a physician and be prepared to transport the victim to a hospital. Provide proper respiratory protection to rescuers entering an unknown atmosphere. Whenever possible, Self-Contained Breathing Apparatus (SCBA) should be used; if not available, use a level of protection greater than or equal to that advised under Protective Clothing.；INGESTION: DO NOT INDUCE VOMITING. If the victim is conscious and not convulsing, give 1 or 2 glasses of water to dilute the chemical and IMMEDIATELY call a hospital or poison control center. Be prepared to transport the victim to a hospital if advised by a physician. If the victim is convulsing or unconscious, do not give anything by mouth, ensure that the victim's airway is open and lay the victim on his/her side with the head lower than the body. DO NOT INDUCE VOMITING. IMMEDIATELY transport the victim to a hospital. (NTP, 1992)

Suitable extinguishing media: Use water spray, alcohol-resistant foam, dry chemical or carbon dioxide.

Advice for firefighters: Wear self-contained breathing apparatus for firefighting if necessary.

ACCIDENTAL RELEASE MEASURES: Personal precautions, protective equipment and emergency procedures: Use personal protective equipment. Avoid dust formation. Avoid breathing vapors, mist or gas. Ensure adequate ventilation. Avoid breathing dust. Environmental precautions: Prevent further leakage or spillage if safe to do so. Do not let product enter drains. Discharge into the environment must be avoided. Methods and materials for containment and cleaning up: Pick up and arrange disposal without creating dust. Sweep up and shovel. Keep in suitable, closed containers for disposal.

SMALL SPILLS AND LEAKAGE: Should a spill occur while you are handling this chemical, FIRST REMOVE ALL SOURCES OF IGNITION, then you should dampen the solid spill material with ethanol and transfer the dampened material to a suitable container. Use absorbent paper dampened with ethanol to pick up any remaining material. Seal the absorbent paper, and any of your clothes, which may be contaminated, in a vapor-tight plastic bag for eventual disposal. Solvent wash all contaminated surfaces with ethanol followed by washing with a soap and water solution. Do not reenter the contaminated area until the Safety Officer (or other responsible person) has verified that the area has been properly cleaned.；STORAGE PRECAUTIONS: You should store this material in a refrigerator. (NTP, 1992)

SRP: Recycle any unused portion of the material for its approved use or return it to the manufacturer or supplier. Ultimate disposal of the chemical must consider: the material's impact on air quality; potential migration in air, soil or water; effects on animal, aquatic and plant life; and conformance with environmental and public health regulations. If it is possible or reasonable use an alternative chemical product with less inherent propensity for occupational harm/injury/toxicity or environmental contamination.

Product: Offer surplus and non-recyclable solutions to a licensed disposal company. Contact a licensed professional waste disposal service to dispose of this material; Contaminated packaging: Dispose of as unused product.

Sweep spilled substance into covered containers. If appropriate, moisten first to prevent dusting. Carefully collect remainder. Then store and dispose of according to local regulations. Do NOT let this chemical enter the environment. Personal protection: particulate filter respirator adapted to the airborne concentration of the substance.

ACCIDENTAL RELEASE MEASURES: Personal precautions, protective equipment and emergency procedures: Use personal protective equipment. Avoid dust formation. Avoid breathing vapors, mist or gas. Ensure adequate ventilation. Avoid breathing dust. Environmental precautions: Prevent further leakage or spillage if safe to do so. Do not let product enter drains. Discharge into the environment must be avoided.

Precautions for safe handling: Avoid contact with skin and eyes. Avoid formation of dust and aerosols. Further processing of solid materials may result in the formation of combustible dusts. The potential for combustible dust formation should be taken into consideration before additional processing occurs. Provide appropriate exhaust ventilation at places where dust is formed.

Appropriate engineering controls: Handle in accordance with good industrial hygiene and safety practice. Wash hands before breaks and at the end of workday.

Stable under recommended storage conditions.

For more Preventive Measures (Complete) data for 1,2,3-Benzotriazole (6 total), please visit the HSDB record page.

Benzotriazole (BTR), an emerging class of environmental pollutant, is widely used in industrial applications and household dishwashing agents. Despite the reported toxicity of BTR to aquatic organisms, little is known about its effects on terrestrial invertebrates. Copper (Cu) accumulates in agricultural soils receiving urban waste products, fertilizers, fungicides, and urban sewage. In this study, two different types of bioassays (acute toxicity test and behavioral toxicity test) were performed to evaluate the toxicity of Cu and BTR, both singly and together, on the earthworm (Eisenia fetida) in artificial soil. The results of avoidance behavior tests showed that the EC50 (48 hr) values for Cu and BTR were 1.47 and 0.46 mmol/kg, respectively. The results of the acute toxicity tests showed that the LC50 (7 d) and LC50 (14 d) of Cu in earthworms were 9.19 and 5.28 mmol/kg, respectively, and the LC50 (7 d) and LC50 (14 d) of BTR were 2.43 and 1.76 mmol/kg, respectively. Toxicity analysis demonstrated that the binary BTR and Cu mixture had predominantly antagonistic effects on the avoidance behavior and survival of earthworms. The Cu2+ activities and mortality of earthworms decreased significantly with increasing concentrations of BTR, while the solid-liquid distribution coefficient of Cu increased. These results indicated that the presence of BTR can reduce the toxicity as well as the bioavailability of Cu in soil with both BTR and Cu.

As an emerging contaminant, 1-H-benzotriazole (1H-BTR) has been detected in the engineered and natural aquatic environments, which usually coexists with heavy metals and causes combined pollution. In the present study, wild-type and transgenic zebrafish Danio rerio were used to explore the acute toxicity as well as the single and joint hepatotoxicity of cadmium (Cd) and 1H-BTR. Although the acute toxicity of 1H-BTR to zebrafish was low, increased expression of liver-specific fatty acid binding protein was observed in transgenic zebrafish when the embryos were exposed to 5.0 uM of 1H-BTR for 30 days. Besides, co-exposure to 1H-BTR not only reduced the acute toxic effects induced by Cd, but also alleviated the Cd-induced liver atrophy in transgenic fish. Correspondingly, effects of combined exposure to 1H-BTR on the Cd-induced expressions of several signal pathway-related genes and superoxide dismutase and glutathione-s-transferase proteins were studied. Based on the determination of Cd bioaccumulation in fish and the complexing stability constant (beta) of Cd-BTR complex in solution, the detoxification mechanism of co-existing 1H-BTR on Cd to the zebrafish was discussed.

EC50; Species: Eisenia fetida (Earthworm) body weight 300-400 mg; Conditions: Artificial soil (70% industrial sand, 20% kaolin clay, and 10% finely ground peat), pH 6.0 +/- 0.5; Concentration: 0.46 mmol/kg for 48 hr (95% confidence interval: 0.41-0.51 mmol/kg); Effect: avoidance behavior. /99% purity/

LC50; Species: Eisenia fetida (Earthworm) body weight 300-400 mg; Conditions: Artificial soil (70% industrial sand, 20% kaolin clay, and 10% finely ground peat), pH 6.0 +/- 0.5; Concentration: 2.43 mmol/kg for 7 days (95% confidence interval: 2.21-2.71 mmol/kg) /99% purity/

LC50; Species: Eisenia fetida (Earthworm) body weight 300-400 mg; Conditions: Artificial soil (70% industrial sand, 20% kaolin clay, and 10% finely ground peat), pH 6.0 +/- 0.5; Concentration: 1.76 mmol/kg for 14 days (95% confidence interval: 1.69-1.82 mmol/kg) /99% purity/

LC50; Species: Dugesia japonica (Flatworm) body length 0.8 cm; Conditions: freshwater, static, 25 °C; Concentration: 171100 ug/L for 24 hr (95% confidence interval: 161700-181100 ug/L) /100% purity/

For more Ecotoxicity Values (Complete) data for 1,2,3-Benzotriazole (7 total), please visit the HSDB record page.

TERRESTRIAL FATE: Based on a classification scheme(1), an experimentally derived Koc range of 10 to 500(2,3) indicates that 1,2,3-benzotriazole is expected to have very high to moderate mobility in soil(SRC). Sorption in soil is influenced by surface complexation of the neutral species, pH, ion exchange(3) and metal content in the soil(2). The pKa of 1,2,3-benzotriazole is 8.37(4), indicating that this compound will exist partially in anion form in the environment and anions generally do not adsorb more strongly to soils containing organic carbon and clay than their neutral counterparts(5). Results of sorption tests at pH 5.2 to 8.6 indicated that 1,2,3-benzotriazole existed primarily as the neutral species(3). Volatilization of 1,2,3-benzotriazole from moist soil surfaces is not expected to be an important fate process(SRC) given an estimated Henry's Law constant of 1.5X10-7 atm-cu m/mole(SRC), using a fragment constant estimation method(6). A 2% of theoretical BOD using activated sludge in the Japanese MITI test(7) indicates that 1,2,3-benzotriazole is not readily biodegradable(SRC). The results of other screening tests and soil degradation studies indicated that 1,2,3-benzotriazole is stable with regard to biodegradation in soils under environmental conditions with a half-life >180 days(8). A compilation of biodegradation rate constants in soil and groundwater for 1,2,3-benzotriazole has a range of 0.001 to 0.016/day(9) which has a corresponding half-life range of 43 to 693 days(SRC). Anaerobic soil column studies(10) suggest biodegradation may occur faster under anaerobic conditions(SRC).

AQUATIC FATE: Based on a classification scheme(1), an experimentally derived Koc range of 10 to 500(2,3) indicates that 1,2,3-benzotriazole may have some adsorption to suspended solids and sediment(SRC). Sorption is influenced by surface complexation of the neutral species, pH, ion exchange(3) and metal content in the sediment(2). Volatilization from water surfaces is not expected(4) based upon an estimated Henry's Law constant of 1.5X10-7 atm-cu m/mole(SRC), developed using a fragment constant estimation method(5). According to a classification scheme(6), a BCF range of 1.1 to 15 measured in carp (Cyprinus carpio), suggests bioconcentration in aquatic organisms is low(SRC). A 2% of theoretical BOD using activated sludge in the Japanese MITI test(7) indicates that 1,2,3-benzotriazole is not readily biodegradable(SRC). The results of other screening tests indicate that 1,2,3-benzotriazole is stable with regard to biodegradation based on little or no observed degradation over periods of 4 weeks or longer(8). 1,2,3-Benzotriazole is not expected to undergo hydrolysis in the environment due to the lack of functional groups that hydrolyze under environmental conditions(4). 1,2,3-Benzotriazole is slowly photodecomposed by irradiation at 300 nm in aqueous solutions(9).

ATMOSPHERIC FATE: According to a model of gas/particle partitioning of semivolatile organic compounds in the atmosphere(1), 1,2,3-benzotriazole, which has an estimated vapor pressure of 2.5X10-5 mm Hg at 25 °C(SRC), determined from a fragment constant method(2), will exist in both the vapor and particulate phases in the ambient atmosphere. Vapor-phase 1,2,3-benzotriazole is degraded in the atmosphere by reaction with photochemically-produced hydroxyl radicals(SRC); the half-life for this reaction in air is estimated to be 16 days(SRC), calculated from its rate constant of 1.0X10-12 cu cm/molecule-sec at 25 °C(SRC) that was derived using a structure estimation method(2). Particulate-phase 1,2,3-benzotriazole may be removed from the air by wet or dry deposition(SRC). 1,2,3-Benzotriazole absorbs at wavelengths >290 nm(3) and, therefore, may be susceptible to direct photolysis by sunlight(SRC).

IDENTIFICATION AND USE: 1,2,3-Benzotriazole (BT) is a white to light tan, crystalline powder. It is used as photographic restrainer and a chemical intermediate. It is also a corrosion inhibitor in industrial water treatment, and in the treatment of bronze disease in metal fine art conservation. HUMAN STUDIES: A report showed that two metal workers developed contact dermatitis from exposure to lubricating oil that contained BT. ANIMAL STUDIES: In a test for primary skin irritation and sensitization on guinea pigs, BT was at most mildly irritating in concentrations up to 50% in ethanol and was not a sensitizer. The dry powder is severely irritating to rabbit eyes (0.1 mL unwashed) but prompt water washing reduces irritation considerably. BT was positive in the Salmonella typhimurium and Escherichia coli mutagenicity assays. ECOTOXICITY STUDIES: This chemical has been widely detected in aquatic environments and shows some degree of environmental persistence. BT exposure can negatively affect endocrine systems and can result in neurotoxicity in fish. BT demonstrated hepatotoxicity and neurotoxicity in Chinese rare minnow. In female marine medaka, exposure to 0.01 mg/L BT caused notable changes in expression levels of vitellogenin, CYP1A1 and CYP19a. In vitro assays conducted using a recombinant yeast (anti-) estrogen assay indicated that BT possessed clear antiestrogenic properties. BT metabolism by plants could mask the presence of BT contamination in the environment. Furthermore, BT-derived metabolites are structurally related to plant auxin hormones.

/AQUATIC SPECIES/ Benzotriazole (BT) and its associated derivatives are used ubiquitously in industrial processes, and can be detected in indoor temperature coolants and in chemicals designed to inhibit corrosion. This chemical has been widely detected in aquatic environments and shows some degree of environmental persistence. Evidence has shown that BT exposure can negatively affect endocrine systems and can result in neurotoxicity in fish. However, no study has examined whether this chemical exhibits hepatotoxicity in fish, and if so, what are the underlying mechanism associated with the damage. To address this knowledge gap, we measured the liver proteome of adult male Chinese rare minnow (Gobiocypris rarus) exposed to either 0.05, 0.5, or 5 mg/L BT for 28 days. Overall, 17 proteins were induced and 9 were reduced in abundance following BT treatment (ratio > 1.5, p < 0.05). Pathway analysis revealed that cellular processes affected by BT included xenobiotic clearance, oxidative stress response, apoptosis, and translation. Moreover, transcripts related to these toxic pathways were also significantly affected by BT. In addition, rare minnows exposed to BT showed signs of hypertrophy of hepatocytes, nuclei pyknosis, and higher levels of cellular vacuolization compared to the controls, thus these early proteomic responses in the liver may be related to pathology (i.e. adverse outcome pathway). Our data demonstrate that BT dysregulates molecular responses in the liver and tissue pathology indicative of damage. This study provides new insight into BT hepatotoxicity in Chinese rare minnow.

/AQUATIC SPECIES/ Benzotriazoles (BZTs) are ubiquitous aquatic contaminants used in a wide range of industrial and domestic applications from aircraft deicers to dishwasher tablets. Acute toxicity has been reported in aquatic organisms for some of the BZTs but their mode of action remains unknown. The objectives of this study were to evaluate the transcriptomic response of D. magna exposed to sublethal doses of 1H-benzotriazole (BTR), 5-methyl-1H-benzotriazole (5MeBTR) and 5-chloro-1H-benzotriazole (5ClBTR) using RNA-sequencing and quantitative real-time PCR. Cellular and life-history endpoints (survival, number of neonates, growth) were also investigated. Significant effects on the molting frequency were observed after 21-day exposure to 5MeBTR and 5ClBTR. No effects on molting frequency were observed for BTR but RNA-seq results indicated that this BZT induced the up-regulation of genes coding for cuticular proteins, which could have compensated the molting disruption. Molting in cladocerans is actively controlled by ecdysteroid hormones. Complementary short-term temporal analysis (4- and 8-day exposure) of the transcription of genes related to molting and hormone-mediated processes indicated that the three compounds had specific modes of action. BTR induced the transcription of genes involved in 20-hydroxyecdysone synthesis, which suggests pro-ecdysteroid properties. 5ClBTR exposure induced protein activity and transcriptional levels of chitinase enzymes, associated with an impact on ecdysteroid signaling pathways, which could explain the decrease in molt frequency. Finally, 5MeBTR seemed to increase molt frequency through epigenetic processes. Overall, results suggested that molting effects observed at the physiological level could be linked to endocrine regulation impacts of BZTs at the molecular level.

EPA has released the Interactive Chemical Safety for Sustainability (iCSS) Dashboard. The iCSS Dashboard provides an interactive tool to explore rapid, automated (or in vitro high-throughput) chemical screening data generated by the Toxicity Forecaster (ToxCast) project and the federal Toxicity Testing in the 21st century (Tox21) collaboration. /The title compound was tested by ToxCast and/or Tox21 assays/[USEPA; ICSS Dashboard Application; Available from, as of November 16, 2017: http://actor.epa.gov/dashboard/]

The following link will take the user to the National Toxicology Program (NTP) Test Status of Agents Search page, which tabulates the results and current status of tests such as "Short-Term Toxicity Studies", "Long-term Carcinogenicity Studies", "Developmental Studies", "Genetic Toxicology Studies", etc., performed with this chemical. Testing status for 1,2,3-benzotriazole is available.[Available from, as of November 3, 2017: https://ntpsearch.niehs.nih.gov/?e=True&ContentType=Testing+Status]

Monitoring at a municipal wastewater treatment plant in Berlin, Germany detected influent and effluent 1,2,3-benzotriazole concentrations of 11.9 and 9.6 ug/L respectively(1). Sewage sludge collected from wastewater treatment plants along the Songhua River, China contained 1,2,3-benzotriazole concentrations of 17.2-198 ng/g dry wt(2). In 2010, effluents from 90 European wastewater treatment plants (WWTPs) were analyzed for 156 polar organic chemical contaminants, and 1,2,3-benzotriazole was detected at a frequency of 97% with average, median and maximum concentrations of 6.3, 2.7 and 221 ug/L respectively(3). The minimum, maximum and median concentrations of 1,2,3-benzotriazole detected in the effluent of a WWTP in Switerland (12 samples) were 2.7, 6.0 and 4.3 mg/L respectively(4). Wastewater sampled from a WWTP in Brandenburg, Germany contained a 1,2,3-benzotriazole concentrations of 34 ug/L(5).

1,2,3-Benzotriazole Freundlich adsorption isotherms measured from topsoil and subsoil collected from the Moreppen, Oslo Norway airport were 4.26 and 0.2-0.4 respectively(1); based on respective soil organic content of 0.82 and 0.10%(1), Koc values of 200 to 520 can be calculated(SRC). Using five Eurosoil types (pH range of 5.2 to 8.6, organic content range of 0.33 to 1.85%), 1,2,3-benzotriazole had measured Kd values ranging from about 0.1 to 0.8(2) which correspond to a Koc range of about 10 to 90(SRC). A measured Koc value 49 has also been reported(3). According to a classification scheme(4), these Koc values suggest that 1,2,3-benzotriazole is expected to have very high to moderate mobility in soil. Sorption in soil is influenced by surface complexation of the neutral species, pH, ion exchange(2) and metal content in the soil(1). The pKa of 1,2,3-benzotriazole is 8.37(5), indicating that this compound will exist partially in anion form in the environment and anions generally do not adsorb more strongly to soils containing organic carbon and clay than their neutral counterparts(6). Results of sorption tests at pH 5.2 to 8.6 indicated that 1,2,3-benzotriazole existed primarily as the neutral species(2). Sorption studies in a column using aquifer sediments determined a Koc value of 81(7).

Chemical: 1H-Benzotriazole

Chemical: 2H-Benzotriazole

1H-Benzotriazole is listed on the EPA's Chemical Data Reporting (CDR) system. Manufacturers and importers of 1H-Benzotriazole are required to report information about their production and use of this chemical to the EPA under the Toxic Substances Control Act (TSCA). (40 eCFR Part 711)

TP - indicates a substance that is the subject of a proposed TSCA section 4 test rule.

Status: Active Update: 20-10-2022 https://echa.europa.eu/registration-dossier/-/registered-dossier/14234；Status: Active Update: 20-12-2018 https://echa.europa.eu/registration-dossier/-/registered-dossier/27455

Benzotriazole: Does not have an individual approval but may be used under an appropriate group standard

Pursuant to section 8(d) of TSCA, EPA promulgated a model Health and Safety Data Reporting Rule. The section 8(d) model rule requires manufacturers, importers, and processors of listed chemical substances and mixtures to submit to EPA copies and lists of unpublished health and safety studies. 1,2,3-Benzotriazole is included on this list. Effective date: 4/13/89; Sunset date: 12/19/95.