ヤヌス グリーン B

使用Janus Green B is a stain that interacts with DNA and has been used for histology studies including mitochondrial staining, in which its oxidation and reduction reveals alterations to the electron transfer chain. The stain has been utilized in studies for effective and rapid staining of insect chordotonal organs and peripheral nerves, where the nerves appear dark blue in comparison to non stained tissue. With the use of Janus Green B, rabbit lymphatic vessel studies demonstrate that threadlike structures within the lymphatic vessels, known as Bonghan ducts, contain a high density of mitochondria in their cells.

純化方法The dye dissolves in H2O to give a bluish violet solution which becomes colourless when made 10M in NaOH. It dissolves in EtOH to give a blue-violet colour, filter from insoluble material, then add dry Et2O whereby the dye separates out leaving a small amount of blue colour in solution. Filter off the solid and dry it in a vacuum. Store it in a dark bottle. [Colour Index Vol 4, 3rd edn, 4015 1971.]