N-[トリス(ヒドロキシメチル)メチル]グリシン
N-[トリス(ヒドロキシメチル)メチル]グリシン · N-[1,1-ビス(ヒドロキシメチル)-2-ヒドロキシエチル]グリシン · トリシン · N-[2-ヒドロキシ-1,1-ビス(ヒドロキシメチル)エチル]グリシン · カルボキシメチルアミノメチリジントリメタノール · 1,3-ジヒドロキシ-2-(ヒドロキシメチル)プロパン-2-イルアミノ酢酸 · N-[1,3-ジヒドロキシ-2-(ヒドロキシメチル)プロパン-2-イル]グリシン · N-トリス(ヒドロキシメチル)メチルグリシン
![N-[トリス(ヒドロキシメチル)メチル]グリシン の分子構造式](/img/chemical/79784.png)
識別子と物理属性
| CAS No. | 5704-04-1 |
|---|---|
| 分子式 | C6H13NO5 |
| 平均分子量 | 179.17 g/mol |
| 単同位体質量 | 179.07937252 g/mol |
| SMILES | O=C(O)CNC(CO)(CO)CO |
| InChIKey | SEQKRHFRPICQDD-UHFFFAOYSA-N |
| PubChem CID | 79784 |
| ChEBI | CHEBI:39063 |
| EC No. | 227-193-6 |
| DTXSID | DTXSID1063982 |
| 現地名 | N-[トリス(ヒドロキシメチル)メチル]グリシン |
化学・物理的性質
| XLogP | -5.1 |
|---|---|
| 極性表面積 | 110 |
| 水素結合供与体数 | 5 |
| 水素結合受容体数 | 6 |
| 回転可能結合数 | 6 |
| 重原子数 | 12 |
| 形式電荷 | 0 |
| 複雑度 | 137 |
| Physical Description | Solid[Merck Index] White crystals[Sigma-Aldrich MSDS] |
| 融点 | 186-188 °C (dec.) |
| 沸点 | 311.69°C (rough estimate) |
| 密度 | 1.05 g/mL at 20 °C |
| 蒸気圧 | 0.002-0.004Pa at 20-25℃ |
| 屈折率 | 1.4240 (estimate) |
| 貯蔵条件 | Keep in dark place,Inert atmosphere,Room temperature |
| 溶解度 | H2O: 4M at20℃、透明、無色溶ける |
| 外観・状態 | 結晶性 |
| 色 | 無色透明 (40% (w/w) in H2O) 溶液 |
| 臭い | 無臭 |
| pH | 4.0-6.0 (1M in H2O) |
| pKa（酸解離定数） | 8.1(at 25℃) |
| 由来生物 | synthetic |
| Merck | 14,9651 |
| BRN | 1937804 |
| 安定性 | 安定。 |
| InChI | 1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12) |
| InChIKey | SEQKRHFRPICQDD-UHFFFAOYSA-N |
| SMILES | OCC(CO)(CO)NCC(O)=O |
| LogP（分配係数） | -2.87 at 25℃ |
| CAS データベース | 5704-04-1(CAS DataBase Reference) |
| EPAの化学物質情報 | Glycine, N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]- (5704-04-1) |
| Absorption | ≤0.05 at 280 in H2O at 1M≤0.1 at 260 in H2O at 1M |
化学的特性
White/clear crystalline powder
安全性と法規制
| GHS 分類 | This chemical does not meet GHS hazard criteria for 97.6% (121 of 124) of all reports.Not ClassifiedReported as not meeting GHS hazard criteria by 121 of 124 companies (only 2.4% companies provided GHS information). For more detailed information, please visit ECHA C&L website.Aggregated GHS information provided per 124 reports by companies from 4 notifications to the ECHA C&L Inventory.Reported as not meeting GHS hazard criteria per 121 of 124 reports by companies.There are 3 notifications provided by 3 of 124 reports by companies with hazard statement code(s).Information may vary between notifications depending on impurities, additives, and other factors. The percentage value in parenthesis indicates the notified classification ratio from companies that provide hazard codes. Only hazard codes with percentage values above 10% are shown. For more detailed information, please visit ECHA C&L website. |
|---|---|
| 規制情報 | Chemical: Glycine, N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]-Status: Active Update: 02-03-2021 https://echa.europa.eu/registration-dossier/-/registered-dossier/25731Glycine, N-2-hydroxy-1,1-bis(hydroxymethyl)ethyl-: Does not have an individual approval but may be used under an appropriate group standard |
| その他の安全情報 | Evaluation - Chemicals that are unlikely to require further regulation to manage risks to environment |
| 有害性の概要 | May cause irritation[Sigma-Aldrich MSDS] |
| Hazard Classes and Categories | Not Classified |
| F | 3 |
| 水環境危害等級（WGK） | 3 |
| TSCA 収載状態 | TSCA listed |
| 税関コード | 29052900 |
| 危険文句（R） | 36/37/38 |
| 危険表示 | Xi |
| 安全措置（S） | 26-36-36/37/39 |
| 貯蔵分類 | 13 - Non Combustible Solids |
用途
使用
Good's buffers Tricine is used as an electrophoresis buffer and involved in the separation of low molecular weight proteins and peptides. It operates in the pH range between 7.4 and 8.8. It acts as an effective scavenger of hydroxyl radicals during the study of radiation-induced membrane damage. It is also used in resuspension of cell pellets. It is a zwitterionic amino acid commonly useful in the pH range of 7.4-8.8 and the pKa is 8.05 at 25°C. This buffer is also used as an eluent in asymmetrical flow field-flow fractionation (AFFFF).
一般的な説明
Tricine and its salts are used as biological buffers to control the pH of body fluids in vitro and in vivo. It acts as a precursor for polymers, oxazolones (with carboxylic acids), and oxazolidines (with aldehydes). Trizma can serve as an alkalizing agent in treating acidosis of the blood.
製法・合成法
純化方法
Crystallise Tricine from EtOH and water. [Good et al. Methods Enzymol 24B 53 1968, McGothlin & Jordan Analyt Lett 9 245 1976, Beilstein 18 III/IV 3454.]