4-(2-ヒドロキシエチル)-1-ピペラジンプロパンスルホン酸
4-(2-ヒドロキシエチル)-1-ピペラジンプロパンスルホン酸 · 4-(2-ヒドロキシエチル)ピペラジン-1-(プロパン-1-スルホン酸) · 3-[4-(2-ヒドロキシエチル)ピペラジン-1-イル]-1-プロパンスルホン酸 · 1-(2-ヒドロキシエチル)ピペラジン-4-(1-プロパンスルホン酸) · 4-(2-ヒドロキシエチル)-1-ピペラジンプロパン-1-スルホン酸 · 3-[4-(2-ヒドロキシエチル)ピペラジノ]-1-プロパンスルホン酸 · 4-(2-ヒドロキシエチル)ピペラジン-1-(1-プロパンスルホン酸) · 3-[4-(2-ヒドロキシエチル)-1-ピペラジニル]-1-プロパンスルホン酸
識別子と物理属性
| CAS No. | 16052-06-5 |
|---|---|
| 分子式 | C9H20N2O4S |
| 平均分子量 | 252.33 g/mol |
| 単同位体質量 | 252.11437812 g/mol |
| SMILES | O=S(=O)(O)CCCN1CCN(CCO)CC1 |
| InChIKey | OWXMKDGYPWMGEB-UHFFFAOYSA-N |
| PubChem CID | 85255 |
| ChEBI | CHEBI:42298 |
| EC No. | 240-198-8 |
| DTXSID | DTXSID3066003 |
| 現地名 | 4-(2-ヒドロキシエチル)-1-ピペラジンプロパンスルホン酸 |
化学・物理的性質
| XLogP | -3.6 |
|---|---|
| 極性表面積 | 89.5 |
| 水素結合供与体数 | 2 |
| 水素結合受容体数 | 6 |
| 回転可能結合数 | 6 |
| 重原子数 | 16 |
| 形式電荷 | 0 |
| 複雑度 | 280 |
| 物質分類 | Nitrogen Compounds -> Piperazines |
| Physical Description | Beige powder[Sigma-Aldrich MSDS] |
| 融点 | 237-239 °C(lit.) |
| 密度 | 1.2684 (rough estimate) |
| 屈折率 | 1.6800 (estimate) |
| 貯蔵条件 | Sealed in dry,Room Temperature |
| 溶解度 | H2O: 1M at20℃、透明、無色水に溶ける。 25.2g/L(20℃) |
| 外観・状態 | 粉体・固形 |
| 色 | 白い |
| pH | 5.0-6.5 (25℃, 0.1M in H2O) |
| pKa（酸解離定数） | 8.0(at 25℃) |
| BRN | 3957385 |
| 主な用途 | 診断アッセイの製造 |
| InChI | 1S/C9H20N2O4S/c12-8-7-11-5-3-10(4-6-11)2-1-9-16(13,14)15/h12H,1-9H2,(H,13,14,15) |
| InChIKey | OWXMKDGYPWMGEB-UHFFFAOYSA-N |
| SMILES | OCCN1CCN(CCCS(O)(=O)=O)CC1 |
| CAS データベース | 16052-06-5(CAS DataBase Reference) |
| EPAの化学物質情報 | 1-Piperazinepropanesulfonic acid, 4-(2-hydroxyethyl)- (16052-06-5) |
| Absorption | ≤0.1 at 260 in H2O at 1M≤0.1 at 280 in H2O at 1M |
化学的特性
white crystalline powder
安全性と法規制
| GHS 分類 | This chemical does not meet GHS hazard criteria for 94.2% (65 of 69) of all reports.Not ClassifiedAggregated GHS information provided per 69 reports by companies from 6 notifications to the ECHA C&L Inventory. |
|---|---|
| 規制情報 | Chemical: 1-Piperazinepropanesulfonic acid, 4-(2-hydroxyethyl)-Status: Active Update: 09-09-2021 https://echa.europa.eu/registration-dossier/-/registered-dossier/327991-Piperazinepropanesulphonic acid, 4-(2-hydroxyethyl)-: Does not have an individual approval but may be used under an appropriate group standard |
| 有害性の概要 | Oral LD50 (quail) > 316 mg/kg[RTECS] An eye irritant[Sigma-Aldrich MSDS] See Piperazine and salts. See HEPES. |
| Hazard Classes and Categories | Not Classified |
| 水環境危害等級（WGK） | 3 |
| TSCA 収載状態 | TSCA listed |
| RTECS 番号 | TM2649130 |
| 毒性 | quail,LD50,oral,> 316mg/kg (316mg/kg),Ecotoxicology and Environmental Safety. Vol. 6, Pg. 149, 1982. |
| 税関コード | 29335995 |
| 危険文句（R） | 36/37/38-22 |
| 危険表示 | Xi |
| 安全措置（S） | 24/25-36-26 |
| 危険等級 | IRRITANT |
| 貯蔵分類 | 11 - Combustible Solids |
用途
使用
HEPPS (EPPS) is a commonly used Good's buffer. It is often used as separator in ultrathin isoelectric focusing gels and enhances the resolution of phosphoglucomutase. It is used as a buffering agent in biology and biochemistry. HEPPS is a biological buffer with a useful pH range of 7.3-8.7 and a pKa=8.0 at 25°C. Research on mice with Alzheimer's disease-like amyloid beta plaques has shown that HEPPS can cause the plaques to break up, reversing some of the symptoms in the mice. HEPPS was reported to dissociate amyloid beta oligomers in patients' plasma samples enabling blood diagnosis of Alzheimer's disease.
一般的な説明
HEPPS, or N-(2-Hydroxyethyl)piperazine-N′-(3-propanesulfonic acid), is a zwitterionic buffering agent commonly used in biochemical and molecular biology research. Its mechanism of action is rooted in its ability to maintain a stable pH environment, which is crucial for various enzymatic reactions and protein studies. HEPPS is especially favoured for its buffering capacity in the physiological pH range, typically between pH 6.8 and 8.2, making it ideal for biological assays and cell culture experiments. This compound is extensively utilized in preparing cell culture media, where maintaining a consistent pH is essential for cell viability and growth. Additionally, HEPPS is employed in biochemical assays, such as enzyme kinetics studies, where precise pH control is necessary to accurately measure enzymatic activity. Furthermore, HEPPS has found applications in molecular biology techniques, including nucleic acid purification, PCR (polymerase chain reaction), and protein analysis by SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis). Its compatibility with various biological systems and stability under physiological conditions make HEPPS a valuable tool in research laboratories for maintaining optimal pH conditions and ensuring reliable experimental outcomes in various biochemical and molecular biology studies.