tert-Butyl methyl ether → HCID 9793812、化合物
反応式
[CH:1]([CH:4]([CH:8]([CH3:10])[CH3:9])[C:5]([NH2:7])=[O:6])([CH3:3])C.[CH3:11]OC(C)(C)C>>[CH2:1]([C@@H:4]([CH:8]([CH3:9])[CH3:10])[C:5]([NH2:7])=[O:6])[CH2:3][CH3:11]参加物
3
実験手順
Assay of plasma samples: to test tubes containing 2 μg of the internal standard (diisopropyl acetamide), 0.5 ml of plasma (thawed at room temperature) and 5 ml of tert-butyl methyl ether (TBME) was added. The test tubes were vigorously vortexed for 30 sec and centrifuged at 3000 g for 10 minutes. The organic phase was separated and dried under reduced pressure using a vortex evaporator. Samples were then reconstituted with 150 μl of chloroform and dried under reduced pressure without vortexing. The sample was again reconstituted with 30 μl of chloroform, of which 2 μl were injected into the GC apparatus.
後処理
- additionwas added
- waitcentrifuged at 3000 g for 10 minutes
- customThe organic phase was separated
- customdried under reduced pressure
- customa vortex evaporator
- customdried under reduced pressure without vortexing
出典と根拠
- ORDレコード
- ord-19039cf30e564ed1a92aacb0a5922940
- 出典データセット
- uspto-grants-2005_11
- 特許番号
- US06969732B2
反応ノート
読み込み中…