Phosphate → Glyoxal 2'-deoxyguanosine

HRID 1167597

反応式

HRID 1167597 の反応式
[C@@H:1]1(N2C=CC(=O)NC2=O)[O:8][C@H:5]([CH2:6][OH:7])[C@@H:3]([OH:4])[CH2:2]1.C(C=O)=O.[NH:21]1[C:30](=[O:31])[C:29]2[NH:28][CH:27]=[N:26][C:25]=2[N:24]=[C:22]1[NH2:23].P([O-])([O-])([O-])=O.B(O)(O)O.C(N(CC(O)=O)CC(O)=O)CN(CC([O-])=O)CC([O-])=O.[Na+].[Na+]>>[CH:3]([CH:5]=[O:8])=[O:4].[C@@H:1]1([N:26]2[C:25]3[N:24]=[C:22]([NH2:23])[NH:21][C:30](=[O:31])[C:29]=3[N:28]=[CH:27]2)[O:8][C@H:5]([CH2:6][OH:7])[C@@H:3]([OH:4])[CH2:2]1

参加物

6

反応物 5

HCID 1061Phosphate2 回HCID 7628Boric AcidHCID 13712Deoxyuridine2 回HCID 13020083Ethylenediaminetetraacetic acid disodium saltHCID 136394281Glyoxal guanine2 回

生成物 1

HCID 136394279Glyoxal 2'-deoxyguanosine

実験手順

Glyoxal-2′-deoxyguanosine was synthesized from 2′-deoxyuridine and glyoxal-guanine under the following conditions, by using any one of the aforementioned three sets of enzyme solutions and the Bacillus stearothermophilus JTS 859 enzyme solution obtained in Production Example 2. Specifically, 5 mM phosphate buffer (pH 7) containing 30 mM 2′-deoxyuridine, 30 mM glyoxal-guanine, 100 mM boric acid, and 4 mM disodium ethylenediaminetetraacetate was first prepared. Each (2 mL) of the above four sets of enzyme solutions (which contain bacteria corresponding to 100 mL of the above culture solution) was added to 98 mL of the above phosphate buffer mixture, respectively, resulting in four sets of reaction mixtures. Each reaction mixture was kept at each reaction temperature for 48 hours, allowing the reaction to proceed. The results are shown in Table 3.

後処理

  1. customthe Bacillus stearothermophilus JTS 859 enzyme solution obtained in Production Example 2
  2. customwas first prepared
  3. customrespectively, resulting in four sets of reaction mixtures
  4. customthe reaction

出典と根拠

ORDレコード
ord-8047a8aa0b204e6482842857d3b573ce
出典データセット
uspto-grants-2003_09
特許番号
US06620596B2

関連ノート

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