Acetate → ASIALOGANGLIOSIDE-GM1

HRID 1197403ノート

反応式

HRID 1197403 の反応式
[CH3:1][CH2:2][CH2:3][CH2:4][CH2:5][CH2:6][CH2:7][CH2:8][CH2:9][CH2:10][CH2:11][CH2:12][CH2:13][CH2:14][CH2:15][CH2:16][CH2:17][C:18]([NH:20][C@H:21]([C@H:91]([OH:107])/[CH:92]=[CH:93]/[CH2:94][CH2:95][CH2:96][CH2:97][CH2:98][CH2:99][CH2:100][CH2:101][CH2:102][CH2:103][CH2:104][CH2:105][CH3:106])[CH2:22][O:23][C@@H:24]1[O:29][C@H:28]([CH2:30][OH:31])[C@@H:27]([O:32][C@@H:33]2[O:38][C@H:37]([CH2:39][OH:40])[C@H:36]([O:41][C@@H:42]3[O:47][C@H:46]([CH2:48][OH:49])[C@H:45]([OH:50])[C@H:44]([O:51][C@@H:52]4[O:57][C@H:56]([CH2:58][OH:59])[C@H:55]([OH:60])[C@H:54]([OH:61])[C@H:53]4[OH:62])[C@H:43]3[NH:63][C:64]([CH3:66])=[O:65])[C@H:35]([O:67][C@@]3(C(O)=O)O[C@@H]([C@H](O)[C@H](O)CO)[C@H](NC(C)=O)[C@@H](O)C3)[C@H:34]2[OH:88])[C@H:26]([OH:89])[C@H:25]1[OH:90])=[O:19].C([O-])(=O)C>O>[CH3:1][CH2:2][CH2:3][CH2:4][CH2:5][CH2:6][CH2:7][CH2:8][CH2:9][CH2:10][CH2:11][CH2:12][CH2:13][CH2:14][CH2:15][CH2:16][CH2:17][C:18]([NH:20][CH:21]([CH:91]([OH:107])/[CH:92]=[CH:93]/[CH2:94][CH2:95][CH2:96][CH2:97][CH2:98][CH2:99][CH2:100][CH2:101][CH2:102][CH2:103][CH2:104][CH2:105][CH3:106])[CH2:22][O:23][CH:24]1[O:29][C@H:28]([CH2:30][OH:31])[C@@H:27]([O:32][C@@H:33]2[O:38][C@H:37]([CH2:39][OH:40])[C@H:36]([O:41][C@@H:42]3[O:47][C@H:46]([CH2:48][OH:49])[C@H:45]([OH:50])[C@H:44]([O:51][C@@H:52]4[O:57][C@H:56]([CH2:58][OH:59])[C@H:55]([OH:60])[C@H:54]([OH:61])[C@H:53]4[OH:62])[C@H:43]3[NH:63][C:64]([CH3:66])=[O:65])[C@H:35]([OH:67])[C@H:34]2[OH:88])[C@H:26]([OH:89])[C@H:25]1[OH:90])=[O:19]

参加物

4

反応物 2

HCID 175AcetateHCID 9963963Ganglioside GM1

生成物 1

HCID 6450363ASIALOGANGLIOSIDE-GM1

試薬・触媒・溶媒 1

HCID 962Water溶媒

実験手順

A 10 ml quantity of 0.05% ganglioside GM1 solution, 10 ml of 40 mM acetate buffer (pH 5.0) and 10 ml of sterilized water were mixed together, and to the mixture was added 10 ml of aqueous Isozyme L (3 U/ml), followed by reaction at 37° C. for 8 hours. The reaction mixture was subjected to purification in the same manner as in Example 1, giving about 2.6 mg of asialo GM1 as a white powder. The production of asialo GM1 was confirmed in the same manner as in Example 1.

後処理

  1. additionto the mixture was added 10 ml of aqueous Isozyme L (3 U/ml)
  2. customfollowed by reaction at 37° C. for 8 hours
  3. customThe reaction mixture was subjected to purification in the same manner as in Example 1

出典と根拠

ORDレコード
ord-8521938072454751abdfcfe23566ef80
出典データセット
uspto-grants-1994_01
特許番号
US05275939

反応ノート

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