Panose → Panose

HRID 1353992

反応式

HRID 1353992 の反応式
[CH2:1]([OH:34])[C@H:2]1[O:7][C@H:6]([O:8][CH2:9][C@H:10]2[O:15][C@H:14]([O:16][C@@H:17]([C@H:22]([OH:27])[C@@H:23]([OH:26])[CH:24]=[O:25])[C@H:18]([OH:21])[CH2:19][OH:20])[C@H:13]([OH:28])[C@@H:12]([OH:29])[C@@H:11]2[OH:30])[C@H:5]([OH:31])[C@@H:4]([OH:32])[C@@H:3]1[OH:33]>C([O-])(=O)C>[O:8]=[CH:6][C@@H:5]([C@H:4]([C@@H:3]([C@@H:2]([CH2:1][OH:34])[OH:7])[OH:33])[OH:32])[OH:31].[CH2:1]([OH:34])[C@H:2]1[O:7][C@H:6]([O:8][CH2:9][C@H:10]2[O:15][C@H:14]([O:16][C@@H:17]([C@H:22]([OH:27])[C@@H:23]([OH:26])[CH:24]=[O:25])[C@H:18]([OH:21])[CH2:19][OH:20])[C@H:13]([OH:28])[C@@H:12]([OH:29])[C@@H:11]2[OH:30])[C@H:5]([OH:31])[C@@H:4]([OH:32])[C@@H:3]1[OH:33]

参加物

4

反応物 1

HCID 94448Panose2 回

生成物 2

HCID 94448PanoseHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

試薬・触媒・溶媒 1

HCID 175Acetate溶媒

実験手順

The enzymatic activity of α-isomaltosyl-transferring enzyme can be assayed as follows: Dissolve panose in 100 mm acetate buffer (pH 6.0) to give a concentration of 2% (w/v) f or a substrate solution, add 0.5 ml of an enzyme solution to 0.5 ml of the substrate solution, and keep the mixture at 35° C. for 30 min to proceed the formation of cyclotetrasaccharide from panose. In the reaction system, glucose is formed together with cyclotetrasaccharide from panose. After the reaction, boil the reaction mixture for 10 min to suspend the reaction. Subject the resulting reaction mixture to the glucose oxidase method to quantify the glucose formed in the reaction mixture. In the present invention, one unit activity of α-isomaltosyl-transferring enzyme is defined as the enzyme amount that forms one micromole of glucose per minute under the above enzymatic reaction conditions.

後処理

  1. customto give a concentration of 2% (w/v) f
  2. customat 35° C.
  3. customfor 30 min

出典と根拠

ORDレコード
ord-830cf9066441413db5f6eab4ca695e89
出典データセット
uspto-grants-2007_05
特許番号
US07223570B2

関連ノート

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