Hydrazine → HCID 10240087、化合物

反応式

C1(=O)[N:5]([CH2:6][CH2:7][O:8][C@@H:9]2[C@H:13]([OH:14])[C@@H:12]([CH2:15][O:16][C:17]([C:34]3[CH:39]=[CH:38][CH:37]=[CH:36][CH:35]=3)([C:26]3[CH:31]=[CH:30][C:29]([O:32][CH3:33])=[CH:28][CH:27]=3)[C:18]3[CH:23]=[CH:22][C:21]([O:24][CH3:25])=[CH:20][CH:19]=3)[O:11][C@H:10]2[N:40]2[CH:47]=[C:46]([CH3:48])[C:44](=[O:45])[NH:43][C:41]2=[O:42])C(=O)C2=CC=CC=C12.NN>CO>[CH3:7][OH:8].[OH-:8].[NH4+:5].[NH2:5][CH2:6][CH2:7][O:8][C@@H:9]1[C@H:13]([OH:14])[C@@H:12]([CH2:15][O:16][C:17]([C:34]2[CH:39]=[CH:38][CH:37]=[CH:36][CH:35]=2)([C:18]2[CH:19]=[CH:20][C:21]([O:24][CH3:25])=[CH:22][CH:23]=2)[C:26]2[CH:31]=[CH:30][C:29]([O:32][CH3:33])=[CH:28][CH:27]=2)[O:11][C@H:10]1[N:40]1[CH:47]=[C:46]([CH3:48])[C:44](=[O:45])[NH:43][C:41]1=[O:42]

参加物

実験手順

2′-O-Phthalimidoethyl-5′-O-DMT-5-methyluridine (3, 21.1 g, 0.029 mol) was dissolved in methanol (500 mL). Anhydrous hydrazine (4.9 mL, 0.15 mol) was added and the solution was heated to reflux. TLC after 3 h indicated a complete reaction. The residue was purified by chromatography on silica gel using methanol and then methanol-ammonium hydroxide (98:2) to give 4 (12.4 g, 70%). The material was completely soluble in methylene chloride and traces of silica from leaching of the column were removed by filtration at this stage and reevaporating the solution.