Sulphosuccinimidyl 4-(4-maleimidophenyl)butyrate → HCID 178502833、化合物

HRID 1415662ノート

反応式

HRID 1415662 の反応式
[CH3:1][O:2]/[N:3]=[C:4](\[C:11]([NH:13][C@@H:14]1[C:17](=[O:18])[N:16]2[C:19]([C:32]([O-:34])=[O:33])=[C:20]([CH2:23][S:24][C:25]([C:27]3[O:31][CH:30]=[CH:29][CH:28]=3)=[O:26])[CH2:21][S:22][C@H:15]12)=[O:12])/[C:5]1[N:9]=[C:8]([NH2:10])[S:7][CH:6]=1.[Na+].[C:36]1(=[O:65])[N:40]([C:41]2[CH:46]=[CH:45][C:44]([CH2:47][CH2:48][CH2:49][C:50]([O:52][N:53]3[C:57](=[O:58])[CH2:56][CH:55](S(O)(=O)=O)[C:54]3=[O:63])=[O:51])=[CH:43][CH:42]=2)[C:39](=[O:64])[CH:38]=[CH:37]1>CN(C)C=O>[CH3:1][O:2]/[N:3]=[C:4](\[C:11]([NH:13][C@@H:14]1[C:17](=[O:18])[N:16]2[C:19]([C:32]([OH:34])=[O:33])=[C:20]([CH2:23][S:24][C:25]([C:27]3[O:31][CH:30]=[CH:29][CH:28]=3)=[O:26])[CH2:21][S:22][C@H:15]12)=[O:12])/[C:5]1[N:9]=[C:8]([NH2:10])[S:7][CH:6]=1.[CH2:56]1[C:57](=[O:58])[N:53]([O:52][C:50]([CH2:49][CH2:48][CH2:47][C:44]2[CH:45]=[CH:46][C:41]([N:40]3[C:39](=[O:64])[CH:38]=[CH:37][C:36]3=[O:65])=[CH:42][CH:43]=2)=[O:51])[C:54](=[O:63])[CH2:55]1

参加物

4

反応物 2

HCID 10274690Sulphosuccinimidyl 4-(4-maleimidophenyl)butyrateHCID 23671563Ceftiofur Sodium

生成物 1

HCID 178502833HCID 178502833、化合物

試薬・触媒・溶媒 1

HCID 6228Dimethylformamide溶媒

実験手順

Ceftiofur sodium (82 mg)(The Upjohn Co., Kalamazoo, Mich.) was dissolved in 500 μL PBS-A, and 10 mg sulfosuccinimidyl 4-(p-maleimidophenyl)-butyrate (s-SMPB)(Pierce, Rockford, Ill.) in 20 μL DMF was added. The resulting mixture was stirred for 10-15 min, added dropwise to 30 mg BSA-SH (1.5 mL of a 20 mg/mL stock solution), stirred overnight at ambient temperature, and then dialyzed exhaustively against PBS-A. Similarly, 52 mg ceftiofur sodium in PBS-A and 5 mg s-SMPB in 20 μL N,N-dimethylformamide (DMF) were added dropwise to 20 mg of OVA-SH (1 mL of a 20 mg/mL stock solution), and the mixture was stirred overnight at ambient temperature and dialyzed exhaustively against PBS-B (0.05M sodium phosphate, 0.075M sodium chloride in deionized water, pH 7.2). These conjugates were abbreviated as BSA-S-SMPB-Cef and OVA-S-SMPB-Cef, respectively.

後処理

  1. additionwas added
  2. additionadded dropwise to 30 mg BSA-SH (1.5 mL of a 20 mg/mL stock solution),
  3. stirringstirred overnight at ambient temperature
  4. customdialyzed exhaustively against PBS-A
  5. additionSimilarly, 52 mg ceftiofur sodium in PBS-A and 5 mg s-SMPB in 20 μL N,N-dimethylformamide (DMF) were added dropwise to 20 mg of OVA-SH (1 mL of a 20 mg/mL stock solution), and the mixture
  6. stirringwas stirred overnight at ambient temperature
  7. customdialyzed exhaustively against PBS-B (0.05M sodium phosphate, 0.075M sodium chloride in deionized water, pH 7.2)

出典と根拠

ORDレコード
ord-10c91cd6bc1241bca5e43abef1a76624
出典データセット
uspto-grants-1999_06
特許番号
US05908781

反応ノート

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