関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|
| 反応物 | N-Acetyl-Neuraminic Acid | HCID 439197 | — |
| 反応物 | 2-(acetylamino)-2-deoxy-3-O-beta-D-galactopyranosyl-D-galactopyranose | HCID 441248 | — |
| 反応物 | CMP-Neu5Ac | HCID 448209 | — |
| 反応物 | (2R,3S,4S,5R)-2,3,4,5,6-pentahydroxyhexanal | HCID 3037556 | — |
| 反応物 | beta-D-Galp-(1->3)-beta-D-GlcpNAc | HCID 10177989 | — |
| 反応物 | sodium;[5-acetamido-2-carboxy-4-hydroxy-6-(1,2,3-trihydroxypropyl)oxan-2-yl] [(2R,3S,4R,5R)-5-(4-amino-2-oxopyrimidin-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate | HCID 16760374 | — |
| 生成物 | 3-O-(N-Acetyl-alpha-neuraminosyl)-beta-D-galactopyranose | HCID 5289366 | — |
反応手順
The resulting CMP-sialic acid analogue (which in FIG. 3 is illustrated as the CMP derivative of Neu5Ac, i.e., CMP-Neu5Ac) is then combined with the derivatized βGal(1→3)βGlcNAc-OR compound in the presence of the βGal(1→3/4)βGlcNAc α(2→3)sialyltransferase or the βGal(1→3)GalNAc α(2→3)sialyltransferase under conditions wherein sialic acid is transferred to the 3 position of the galactose to form a αNeu5Ac(2→3)βGal linkage. Suitable conditions, known in the art, include the addition of the sialyltransferase to a mixture of the derivatized βGal(1→3)βGlcNAc-OR compound and of the CMP-sialic acid in a appropriate buffer such as 0.1M sodium cacodylate in appropriate conditions of pH and temperature such as at a pH of 6.5 to 7.5 and a temperature between 25° and 45° C., preferably 35°-40° C., while incubating for 12 hours to 4 days. The resulting sialylated product can be isolated and purified using conventional methodology comprising HPLC, ion exchange-, gel-, reverse-phase- or adsorption chromatography.
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
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| 特許番号 | US05882901 |
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| 出典データセット | uspto-grants-1999_03 |
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| ORD ID | ord-a820dde74a1b4cfbbbac8d5c5da8daa3 |
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| 出典メモ | uspto-grants-1999_03 |
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