化工社化工社 化学情報印刷レポート · 研究参考用
Hydrochloric Acid → 1,4,7-Tris(carboxymethyl)-1,4,7,10-tetraazacyclododecane
関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|---|---|---|
| 反応物 | Hydrochloric Acid | HCID 313 | — |
| 反応物 | Sodium Hydroxide | HCID 14798 | — |
| 反応物 | 1-Ethyl-3-(3'-dimethylaminopropyl)carbodiimide | HCID 15908 | — |
| 反応物 | 3-Amino-1,2-propanediol | HCID 73561 | — |
| 反応物 | n-hydroxysulfosuccinimide sodium salt | HCID 3520574 | — |
| 反応物 | 2-[4,7-Bis(1,4-dicarboxybutyl]-1,4,7,10-tetraazacyclododec-1-yl]hexanoic acid | HCID 66739089 | — |
| 溶媒 | Water | HCID 962 | — |
| 生成物 | 1,4,7-Tris(carboxymethyl)-1,4,7,10-tetraazacyclododecane | HCID 107830 | — |
反応手順
A solution containing 0.26 mg of 3-aminopropane-1,2-diol in 6 μl of water is prepared. The pH is adjusted to 6 with HCl. 0.5 mg of gallium complex of 2-[4,7-bis(1,4-dicarboxybutyl]-1,4,7,10-tetraazacyclododec-1-yl]hexanoic acid are added to the above solution. The pH is again adjusted before adding 0.071 mg of sulfo-NHS and 0.062 mg of EDCI. The pH is checked and adjusted to 6 with 2N NaOH. After an overnight period at AT, the reaction medium is concentrated to approximately 2 ml and then precipitated from 10 ml of ethanol. The solid is filtered off, washed with ethanol and diethyl ether, and then purified on silanized silica RP2, elution being carried out with water only. 0.2 mg of product is obtained.
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 特許番号 | US08926945B2 |
| 出典データセット | uspto-grants-2015_01 |
| ORD ID | ord-367de10a3cab4b43a995523b367e5bc6 |
| 出典メモ | uspto-grants-2015_01 |