Sucrose → (2S,3'R,3aR,4R,4'S,5'R,6R,6'R,7S,7aS)-4-[(1S,2R,3S,5R,6S)-3-amino-2,6-dihydroxy-5-(methylamino)cyclohexyl]oxy-6'-[(1R)-1-amino-2-hydroxyethyl]-6-(hydroxymethyl)spiro[4,6,7,7a-tetrahydro-3aH-[1,3]dioxolo[4,5-c]pyran-2,2'-oxane]-3',4',5',7-tetrol

HRID 14365

反応式

HRID 14365 の反応式
C(O)[C@H]1O[C@H](O[C@]2(CO)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@H](O)[C@@H](O)[C@@H]1O.[CH3:24][NH:25][C@@H:26]1[C@@H:31]([OH:32])[C@H:30]([O:33][C@@H:34]2[O:39][C@H:38]([CH2:40][OH:41])[C@H:37]([OH:42])[C@@H:36]3[O:43][C:44]4([O:50][C@H:49]([C@@H:51]([NH2:54])[CH2:52][OH:53])[C@H:48]([OH:55])[C@@H:47]([OH:56])[C@H:46]4[OH:57])[O:45][C@H:35]23)[C@@H:29]([OH:58])[C@H:28]([NH2:59])[CH2:27]1>>[CH3:24][NH:25][C@H:26]1[C@H:31]([OH:32])[C@@H:30]([O:33][C@H:34]2[O:39][C@H:38]([CH2:40][OH:41])[C@H:37]([OH:42])[C@@H:36]3[O:43][C@:44]4([O:50][C@H:49]([C@H:51]([NH2:54])[CH2:52][OH:53])[C@H:48]([OH:55])[C@H:47]([OH:56])[C@H:46]4[OH:57])[O:45][C@@H:35]23)[C@H:29]([OH:58])[C@@H:28]([NH2:59])[CH2:27]1

参加物

3

反応物 2

HCID 5988SucroseHCID 35766Hygrovetine

生成物 1

HCID 52949613(2S,3'R,3aR,4R,4'S,5'R,6R,6'R,7S,7aS)-4-[(1S,2R,3S,5R,6S)-3-amino-2,6-dihydroxy-5-(methylamino)cyclohexyl]oxy-6'-[(1R)-1-amino-2-hydroxyethyl]-6-(hydroxymethyl)spiro[4,6,7,7a-tetrahydro-3aH-[1,3]dioxolo[4,5-c]pyran-2,2'-oxane]-3',4',5',7-tetrol

実験手順

After completion of the electroshock, 1 ml of Vogel's minimal medium that contained 1.2% sucrose was added to the resultant, and the obtained mixture was then incubated at 30° C. for 2 hours. 200 μl of the obtained solution was applied to and expanded on an agar medium that contained hygromycin B (500 μg/ml). A hygromycin-resistance colony was isolated, and it was then confirmed by PCR whether or not substitution took place in the target locus. In addition, it was also confirmed by the Southern blot method whether or not it contained redundant Hygr gene copies.

後処理

  1. additionwas added to the resultant

出典と根拠

ORDレコード
ord-0abf829f876f400f8a0f7cb628a03d6a
出典データセット
uspto-grants-2010_06
特許番号
US07732208B2

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