Acetate → 4-Nitrophenol

HRID 1442885

反応式

HRID 1442885 の反応式
C([O-])(=O)C.C(C(C(C([O-])=O)O)O)([O-])=O.P([O:19][C:20]1[CH:25]=[CH:24][C:23]([N+:26]([O-:28])=[O:27])=[CH:22][CH:21]=1)(O)(O)=O>>[N+:26]([C:23]1[CH:24]=[CH:25][C:20]([OH:19])=[CH:21][CH:22]=1)([O-:28])=[O:27]

参加物

4

反応物 3

HCID 175AcetateHCID 3784-Nitrophenyl PhosphateHCID 38061142,3-Dihydroxybutanedioate

生成物 1

HCID 9804-Nitrophenol

実験手順

The scales treated by fixation were moved and weighed. After measurement, the scales were put into a 96-well microplate. To each well, 2001 of 100 mM acetate buffer (pH 5.3) containing 20 mM tartrate and 10 mM para-nitrophenol phosphate (substrate) was added. Reaction was performed at 25° C. for 20 minutes and subsequently terminated by the addition of a 3 M sodium hydroxide aqueous solution (50±1). Then, 1501 of the solution after the completion of reaction was transferred to another microplate. The amount of para-nitrophenol (p-NP) produced by TRAP was measured with a spectrophotometer (405 nm). The osteoclast activity was indicated as the amount of p-NP produced by the decomposition of para-nitrophenol phosphate per g of scale per minute.

後処理

  1. additionThe scales treated by fixation
  2. additionwas added
  3. customReaction
  4. customsubsequently terminated by the addition of a 3 M sodium hydroxide aqueous solution (50±1)
  5. customThen, 1501 of the solution after the completion of reaction

出典と根拠

ORDレコード
ord-b6424bfdd7d848098ef4ba7bef299028
出典データセット
uspto-grants-2010_02
特許番号
US07659304B2

関連ノート

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