Phosphate → Ethyl 2-benzyl-3-hydroxypropanoate

HRID 1445310

反応式

HRID 1445310 の反応式
O=C[C@@H]([C@H]([C@@H]([C@@H](CO)O)O)O)O.[CH:13]([CH:15]([CH2:21][C:22]1[CH:27]=[CH:26][CH:25]=[CH:24][CH:23]=1)[C:16]([O:18][CH2:19][CH3:20])=[O:17])=[O:14].C1N=C(N)C2N=CN([C@@H]3O[C@H](COP(OP(OC[C@H]4O[C@@H](N5C=C(C(N)=O)CC=C5)[C@H](O)[C@@H]4O)(O)=O)(O)=O)[C@@H](O)[C@H]3O)C=2N=1.C1C=[N+]([C@@H]2O[C@H](COP(OP(OC[C@H]3O[C@@H](N4C5N=CN=C(N)C=5N=C4)[C@H](OP(O)(O)=O)[C@@H]3O)(O)=O)(O)=O)[C@@H](O)[C@H]2O)C=C(C(N)=O)C=1.P([O-])([O-])([O-])=O>C(OCC)(=O)C.CC(C)=O>[OH:14][CH2:13][CH:15]([CH2:21][C:22]1[CH:23]=[CH:24][CH:25]=[CH:26][CH:27]=1)[C:16]([O:18][CH2:19][CH3:20])=[O:17]

参加物

8

反応物 5

HCID 1061PhosphateHCID 5886NADP nicotinamide-adenine-dinucleotide phosphateHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal2 回HCID 439153NadhHCID 562571Ethyl 2-benzyl-3-oxopropanoate

生成物 1

HCID 562348Ethyl 2-benzyl-3-hydroxypropanoate

試薬・触媒・溶媒 2

HCID 180Acetone溶媒HCID 8857Ethyl Acetate溶媒

反応条件

温度
30 °C

実験手順

A liquid medium (pH 7.0) was prepared so as to contain 4% glucose, 0.3% of yeast extract, 1.3% of KH2PO4, 0.7% of (NH4)2HPO4, 0.01% of NaCl, 0.08% of MgSO4.7H2O, 0.006% of ZnSO4.7H2O, 0.009% of FeSO4.7H2O, 0.0005% of CuSO4.5H2O, and 0.001% of MnSO4.4 to 5 H2O. The liquid medium was dispensed to large test tubes in an amount of 5 ml per each tube and the test tubes were sterilized by steam at 120° C. for 20 minutes. The liquid medium was inoculated each of the microorganisms shown in Table 3 by a platinum loop and incubated with shaking at 30° C. for 2 to 3 days. Microbial cells were centrifugally collected from each culture solution, and washed with water. After ice cold acetone was added, the cells were dried under reduced pressure to obtain acetone dried microbial cells. 5 mg of the acetone dried microbial cells, 2 mg of ethyl 2-formyl-3-phenylpropionate, 10 mg of glucose, 1 mg of NAD (or NADP), 0.5 ml of 0.1M phosphate buffer (pH=6.5) and 0.5 ml of ethyl acetate were added to each test tube with a cap and shaken at 30° C. for 24 ours. Subsequently, the same operation as in Example 9 was performed to determine a conversion rate and optical purity. The results are shown in Table 3.

後処理

  1. customA liquid medium (pH 7.0) was prepared so as
  2. extractionextract
  3. customThe liquid medium was dispensed to large test tubes in an amount of 5 ml per each tube
  4. customMicrobial cells were centrifugally collected from each culture solution
  5. washwashed with water
  6. additionAfter ice cold acetone was added
  7. customthe cells were dried under reduced pressure
  8. customto obtain acetone dried microbial cells
  9. customa cap
  10. stirringshaken at 30° C. for 24 ours

出典と根拠

ORDレコード
ord-0f77f1bb472d45d5949ab3edda8f5cf0
出典データセット
uspto-grants-2010_02
特許番号
US07666659B2

関連ノート

読み込み中…