L-Lysine → L-Leucine
反応式
N[C@H](C(O)=O)CCCCN.N[C@H](C(O)=O)C.N[C@H](C(O)=O)CCCCN.[NH2:27][C@H:28]([C:36]([OH:38])=[O:37])[CH2:29][C:30]1[CH:35]=CC=C[CH:31]=1.N[C@H](C(O)=O)CCCCN>>[NH2:27][C@H:28]([C:36]([OH:38])=[O:37])[CH2:29][CH:30]([CH3:35])[CH3:31]参加物
4
実験手順
These compounds were prepared from the corresponding compounds I-III by removal of protecting groups with HBr in acetic acid. The benzyloxycarbonyl and t-butyloxycarbonyl groups were simultaneously removed by treatment with 30% Hbr in acetic acid containing anisol (10%) for 15 minutes at room temperature. Yields were about 90%. The molar ratios of the constituent amino acid residues and electrophoretic migrations relative to lysine were for compounds IV: Ala/Lys=1.05 (calc. 1.00), RLys =0.55, for compound V: Phe/Lys=1.06 (calc. 1.00), RLys =0.46, for compound VI: Ala/Lys=1.98 (calc. 2.00); RLys =0.51. Incubation of each of the compounds IV, V and VI with leucine aminopeptidase (E.C. 3.4.11.1) produced quantitatively ε(2-aminobenzoyl)-L-lysisne, (RLys =0.26) and the other expected product as judged by high voltage paper electrophoresis using authentic markers.