反応式
[CH:1]1[C:6]([OH:7])=[CH:5][C:4]2[C:8]([CH2:11][CH:12]([NH2:16])[C:13]([OH:15])=[O:14])=[CH:9][NH:10][C:3]=2[CH:2]=1.CS(C)=O.[O:21]=[C:22]1[NH:30][CH:29]=[N:28][C:27]2[N:26]([CH2:31][CH2:32][C:33](OC3C=CC([N+]([O-])=O)=CC=3)=[O:34])[CH:25]=[N:24][C:23]1=2>CC(C)=O>[O:21]=[C:22]1[NH:30][CH:29]=[N:28][C:27]2[N:26]([CH2:31][CH2:32][C:33]([NH:16][CH:12]([CH2:11][C:8]3[C:4]4[C:3](=[CH:2][CH:1]=[C:6]([OH:7])[CH:5]=4)[NH:10][CH:9]=3)[C:13]([OH:15])=[O:14])=[O:34])[CH:25]=[N:24][C:23]1=2参加物
実験手順
100 mg (0.500 mmol) of DL-5-hydroxytryptophan and 3 ml of dry dimethylsulfoxide were placed in a 25 ml round bottom flask equipped with a magnetic stirring bar and stopper. Then 150 mg (0.456 mmol) of 3-(1,6-dihydro-6-oxo-9H-purin-9-yl)propanoic acid, 4-nitrophenyl ester (AIT-0081) were added and the heterogeneous mixture was stirred for 2.5 hours at room temperature in the closed flask. The mixture became completely homogeneous. The reaction mixture was poured into 20 ml of acetone and a semi-solid precipitate was obtained by filtration. The crude product was washed twice with 2 ml of acetone. The solid was resuspended in 10 ml of acetone and the insoluble material was removed by filtration. The acetone solution was evaporated to dryness. The yield of product 2-[[3-(1,6-dihydro-6-oxo-9H-purin-9-yl)-1-oxopropyl]amino]-3-(5-hydroxyl-1H-indol-3-yl)-propionic acid (AIT-0111) was 15 mg. Yield: 7.3% (Purity 93 %).
後処理
- customequipped with a magnetic stirring bar
- customa semi-solid precipitate was obtained by filtration
- washThe crude product was washed twice with 2 ml of acetone
- customthe insoluble material was removed by filtration
- customThe acetone solution was evaporated to dryness
出典と根拠
- ORDレコード
- ord-b67a98265dd44c46b9b57530daeaf694
- 出典データセット
- uspto-grants-1992_02
- 特許番号
- US05091432
関連ノート
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