Z-Gln-ONp → Z-Gln-Thr(tBu)-Ala-Ile-Gly-OMe

HRID 1580887ノート

反応式

HRID 1580887 の反応式
[NH2:1][C@H:2]([C:10]([NH:12][C@H:13]([C:15]([NH:17][C@H:18]([C:23]([NH:25][CH2:26][C:27]([O:29][CH3:30])=[O:28])=[O:24])[C@H:19]([CH2:21][CH3:22])[CH3:20])=[O:16])[CH3:14])=[O:11])[C@@H:3]([CH3:9])[O:4][C:5]([CH3:8])([CH3:7])[CH3:6].[CH:31]1[CH:36]=[CH:35][C:34]([CH2:37][O:38][C:39]([NH:41][C@H:42]([C:48](OC2C=CC([N+]([O-])=O)=CC=2)=[O:49])[CH2:43][CH2:44][C:45]([NH2:47])=[O:46])=[O:40])=[CH:33][CH:32]=1>CN(C)C=O.CCOCC>[NH:41]([C:39]([O:38][CH2:37][C:34]1[CH:35]=[CH:36][CH:31]=[CH:32][CH:33]=1)=[O:40])[C@H:42]([C:48]([NH:1][C@H:2]([C:10]([NH:12][C@H:13]([C:15]([NH:17][C@H:18]([C:23]([NH:25][CH2:26][C:27]([O:29][CH3:30])=[O:28])=[O:24])[C@H:19]([CH2:21][CH3:22])[CH3:20])=[O:16])[CH3:14])=[O:11])[C@@H:3]([CH3:9])[O:4][C:5]([CH3:6])([CH3:8])[CH3:7])=[O:49])[CH2:43][CH2:44][C:45](=[O:46])[NH2:47]

参加物

5

反応物 2

HCID 111333Z-Gln-ONpHCID 131709987H-Thr(tBu)-Ala-Ile-Gly-OMe

生成物 1

HCID 131709986Z-Gln-Thr(tBu)-Ala-Ile-Gly-OMe

試薬・触媒・溶媒 2

HCID 3283Diethyl Ether溶媒HCID 6228Dimethylformamide溶媒

実験手順

4.6 g of the H-Thr(tBu)-Ala-Ile-Gly-OMe described under 28), in 30 ml of dimethylformamide, are mixed with 3.5 g of Z-Gln-ONP and stirred at room temperature until the mixture becomes solid. After allowing to stand overnight the mixture is diluted with ether and the precipitate is filtered off and washed with ether until free of nitrophenol. The protected pentapeptide shows Rf =0.14 in the system chloroform-methanol (95.5 ) in a thin layer chromatogram on silica gel. Melting point: >250°C.

後処理

  1. filtrationthe precipitate is filtered off
  2. washwashed with ether until free of nitrophenol

出典と根拠

ORDレコード
ord-622e71bbc2a04b359b3fe49e45084da6
出典データセット
uspto-grants-1976_01
特許番号
US03934008

反応ノート

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