関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|
| 反応物 | Ethylenediaminetetraacetic Acid | HCID 6049 | — |
| 反応物 | 5,5'-Dithiobis(2-nitrobenzoic Acid) | HCID 6254 | — |
| 反応物 | Acetic Anhydride | HCID 7918 | — |
| 反応物 | Tryptophol | HCID 10685 | — |
| 反応物 | Trisodium Phosphate | HCID 24243 | — |
| 反応物 | Tryptamine hydrochloride | HCID 67652 | — |
| 反応物 | Adenosine 5'-(trihydrogen diphosphate), 3'-(dihydrogen phosphate), P'-((3R)-4-((3-(ethylamino)-3-oxopropyl)amino)-3-hydroxy-2,2-dimethyl-4-oxobutyl) ester | HCID 165363 | — |
| 反応物 | acetyl CoA | HCID 444493 | — |
| 反応物 | D-1,4-Dithiothreitol | HCID 446094 | — |
| 反応物 | [14C]acetylCoA | HCID 73755097 | — |
| 溶媒 | Triethylamine | HCID 8471 | — |
| 生成物 | N-Acetyltryptamine | HCID 70547 | 88% |
反応手順
The following were purchased: acetyl-CoA (Pharmacia Biotech Inc.); tryptamine-HCl, desulfo-CoA, glutathione-agarose, DTNB (Sigma); sodium phosphate, dithiothreitol, guanidinium-HCl, EDTA (Fisher Scientific); tryptophol (Aldrich); and [14C]acetyl-CoA (60 Ci/mol) (NEN Life Science Products). N-acetyltryptamine was synthesized by reacting tryptamine-HCl (500 mg, 2.5 mmol) with acetic anhydride (260 mg, 2.5 mmol) in the presence of excess triethylamine (1.75 ml). After vigorous stirring at room temperature for 50 min, the mixture was partitioned between ethyl acetate (80 ml) and water (70 ml). The organic phase was washed with saturated aqueous NaHCO3 (50 ml), 0.1 M HCl (50 ml), Na2SO4 (anhydride), and the resultant was concentrated in vacuo to afford N-acetyltryptamine as off-white crystals (88% yield). Purity (>95%) was established by TLC and 1H NMR.
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|
| 特許番号 | US05990094 |
|---|
| 出典データセット | uspto-grants-1999_11 |
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| ORD ID | ord-00039a8ac5c842b9bf35fb31b52ea998 |
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| 出典メモ | uspto-grants-1999_11 |
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