Kanamycin A Sulfate → Kanamycin A Sulfate

関与物質

反応条件

反応手順

Simulated kanamycin culture broth is prepared by adding 80 mg of kanamycin sulfate to 160 ml of a glucose bouillon medium, which s sterilized under heating at 120°C for 15 minutes and cooled. The culture broth is then passed through a column of 1.5 cm in diameter packed with 30 g of the selective adsorbing particles obtained in Example 2 at a flowing velocity of 0.5 ml/minute. The column is then washed with water and further eluted with 0.01 N HCl-methanol (1 : 1). Respective active fractions are combined to give the result that 74.4 mg (recovery percentage: 91%) of kanamycin sulfate is obtained in very high purity and yield with 98% decoloration degree and 98% purity.

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