HCID 178511487、化合物 → HCID 178511487、化合物

HRID 1614858ノート

反応式

HRID 1614858 の反応式
[Fe-3:1]([C:12]#[N:13])([C:10]#[N:11])([C:8]#[N:9])([C:6]#[N:7])([C:4]#[N:5])[C:2]#[N:3]>P([O-])([O-])([O-])=O>[Fe-4:1]([C:10]#[N:11])([C:6]#[N:7])([C:2]#[N:3])([C:4]#[N:5])([C:8]#[N:9])[C:12]#[N:13].[Fe-3:1]([C:10]#[N:11])([C:6]#[N:7])([C:2]#[N:3])([C:4]#[N:5])([C:8]#[N:9])[C:12]#[N:13]

参加物

4

反応物 1

HCID 178511487HCID 178511487、化合物2 回

生成物 2

HCID 178511487HCID 178511487、化合物HCID 178511491HCID 178511491、化合物

試薬・触媒・溶媒 1

HCID 1061Phosphate溶媒

実験手順

To determine GOx kinetic constants under the sensing conditions, 600 microL of GOx (0.0127 mM) in 0.5 M phosphate buffer (pH 7.4) and 100 microL of the 0.5 M ferricyanide solution (in 0.5 M phosphate buffer, pH 7.4) were well mixed and maintained at 37 C in a jacketed cuvette. The sample was illuminated with a 785 nm laser and the resultant ferricyanide Raman scattering from the mediator, evident at 2132 cm−1, was collected at 1800 and recorded by a CCD camera. While continuously monitoring the ferricyanide Raman scattering peak, 100 microL of glucose solution (20 mM) was added to the cuvette. The oxidation of glucose by glucose oxidase caused hydrogen peroxide to be produced, which then reduced the free ferricyanide, Fe(CN)63−, to ferrocyanide, Fe(CN)64−, resulting in an intensity loss from ferricyanide scattering. Ferricyanide scattering intensity was normalized to one by dividing the specific intensity by the initial intensity. FIG. 10 shows the change in normalized ferricyanide scattering intensity monitored via Raman scattering at 785 nm at 2132 cm−1 over time after addition of 20 mM glucose. The same experiment was also completed for glucose concentrations of 31, 10 and 5 mM. The ferricyanide response was fit with a normalized ferricyanide concentration, C/Co, modeled using Michaelis-Menton kinetics

後処理

  1. temperaturemaintained at 37 C in a jacketed cuvette
  2. customThe sample was illuminated with a 785 nm laser
  3. customthe resultant ferricyanide Raman scattering from the mediator, evident at 2132 cm−1, was collected at 1800
  4. additionwas added to the cuvette
  5. customto be produced

出典と根拠

ORDレコード
ord-b7da98d1ad804096808cb3b4c3acab84
出典データセット
uspto-grants-2014_07
特許番号
US08765488B2

反応ノート

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