Benzoylarginine ethyl ester → Citrulline

HRID 1676880

反応式

HRID 1676880 の反応式
CN(C1C2C[C@@H]3C(C(=O)C=2C(O)=CC=1)=C(O)[C@@]1(O)[C@H]([C@H](N(C)C)C(O)=C(C(N)=O)C1=[O:18])C3)C.C[C@]1(O)[C@@H]2C(=C(O)[C@]3(O)C(=O)C(C(N)=O)=C(O)[C@@H](N(C)C)[C@@H]3C2)C(=O)C2C(O)=CC=C(Cl)C1=2.C[C@@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](NC(N)=N)[C@@H](O)[C@@H]2NC(N)=N)[C@H](O[C@@H]2O[C@@H](CO)[C@H](O)[C@@H](O)[C@@H]2NC)[C@@]1(O)C=O.CC[O:109][C:110]([C@@H:112]([NH:120]C(C1C=CC=CC=1)=O)[CH2:113][CH2:114][CH2:115][N:116]=[C:117](N)[NH2:118])=[O:111]>>[NH2:120][C@H:112]([C:110]([OH:109])=[O:111])[CH2:113][CH2:114][CH2:115][NH:116][C:117]([NH2:118])=[O:18]

参加物

5

反応物 4

HCID 123880Benzoylarginine ethyl esterHCID 178482881HCID 178482881、化合物HCID 178484163HCID 178484163、化合物HCID 178484872HCID 178484872、化合物

生成物 1

HCID 9750Citrulline

実験手順

Inhibition constants were determined for minocycline, chlortetracycline, and streptomycin using standard kinetic analyses. Briefly, the compounds were incubated in Reaction Buffer containing various concentrations of BAEE (0-20 mM) for 10 minutes at 37° C. PAD4 (0.2 μM) was then added to initiate the reaction and allowed to proceed for 6 additional minutes. Reactions were then quenched by flash freezing in liquid N2 and the amount of Cit produced was quantified. The data obtained for streptomycin, minocycline and chlortetracycline were fit to equations 2 and 3,

後処理

  1. additionPAD4 (0.2 μM) was then added
  2. customthe reaction
  3. customReactions were then quenched by flash

出典と根拠

ORDレコード
ord-3a2d3b4530144744a84496a8d9c05e64
出典データセット
uspto-grants-2011_08
特許番号
US08007996B2

関連ノート

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