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Water → Retinoyl beta-glucuronide
関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|---|---|---|
| 反応物 | Water | HCID 962 | — |
| 反応物 | Glucuronic Acid | HCID 65041 | — |
| 反応物 | Sodium Bicarbonate | HCID 516892 | — |
| 反応物 | (2E,4E,6E,8E)-3,7-dimethyl-9-(2,6,6-trimethylcyclohexen-1-yl)nona-2,4,6,8-tetraenoyl fluoride | HCID 5378594 | — |
| 溶媒 | Propan-2-one--water (1/1) | HCID 18366337 | — |
| 生成物 | Retinoyl beta-glucuronide | HCID 5281877 | — |
反応手順
Retinoyl fluoride can be mixed with commercially available glucuronic acid. The reaction is carried out in acetone/water to facilitate the solubility of the water-insoluble retinoyl fluoride and the water-soluble glucuronic acid. The reaction takes place in slightly alkaline pH and hence sodium bicarbonate is used in the reaction mixture. Stirring the mixture at room temperature for 20-24 hours followed by acidification of the solution and extraction with ethyl acetate yields retinoyl glucuronide as the major product. Pure all-trans retinoyl β-glucuronide can be obtained by a combination of column and high-performance liquid chromatography (HPLC).
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 特許番号 | US04855463 |
| 出典データセット | uspto-grants-1989_08 |
| ORD ID | ord-6187d176f8dd41d0a6a3ec766ba6771b |
| 出典メモ | uspto-grants-1989_08 |