関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|
| 反応物 | 2-Oxoglutaric Acid | HCID 51 | — |
| 反応物 | N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid | HCID 81831 | — |
| 反応物 | L-Proline | HCID 145742 | — |
| 反応物 | L-Ascorbic Acid | HCID 54670067 | — |
| 生成物 | cis-3-Hydroxy-L-proline | HCID 7098652 | — |
反応手順
The cells, the treated cells or the enzyme preparation are added to 240 mM TES [N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid] buffer containing 12 mM L-proline, 24 mM 2-ketoglutaric acid, 4 mM ferrous sulfate and 8 mM L-ascorbic acid to make 250 μl in total. The mixture is kept at 35° C. for 10 minutes. The reaction mixture is heated at 100° C. for 2 minutes to stop the reaction, and the amount of cis-3-hydroxy-L-proline produced in the reaction mixture is determined by HPLC.
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|
| 特許番号 | US05962292 |
|---|
| 出典データセット | uspto-grants-1999_10 |
|---|
| ORD ID | ord-3f8bd898d746464fba35b245cbf3f31d |
|---|
| 出典メモ | uspto-grants-1999_10 |
|---|
