C(O[C:4](=[O:37])[CH2:5][CH2:6][CH2:7][O:8][C:9]1[CH:36]=[CH:35][C:12]([CH2:13][C@@H:14]([C:28]([O:30][C:31]([CH3:34])([CH3:33])[CH3:32])=[O:29])[NH:15][C:16]2[N:20]([CH2:21][C:22]3[CH:27]=[CH:26][CH:25]=[CH:24][CH:23]=3)[N:19]=[N:18][N:17]=2)=[CH:11][CH:10]=1)C.[NH2:38][C:39]1[NH:40][CH2:41][CH2:42][CH2:43][N:44]=1>C(Cl)Cl>[O:37]=[C:4]([NH:38][C:39]1[NH:44][CH2:43][CH2:42][CH2:41][N:40]=1)[CH2:5][CH2:6][CH2:7][O:8][C:9]1[CH:10]=[CH:11][C:12]([CH2:13][C@@H:14]([C:28]([O:30][C:31]([CH3:32])([CH3:34])[CH3:33])=[O:29])[NH:15][C:16]2[N:20]([CH2:21][C:22]3[CH:27]=[CH:26][CH:25]=[CH:24][CH:23]=3)[N:19]=[N:18][N:17]=2)=[CH:35][CH:36]=1The ester 5-1 (150 mg) is mixed with 2-amino-1,4,5,6-tetrahydropyrimidine (42 mg) in 5 ml of CH2Cl2 and agitation is carried out at ambient temperature for 24 hours. Evaporation is then carried out under reduced pressure and the dry extract obtained is purified by chromatography, eluting with a CH2Cl2/MeOH/water/AcOH mixture 90/10/1/1. 40 mg of the expected product 5-2 is obtained.
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