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Alpha-Ketoglutarate → Oxalacetate
関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|---|---|---|
| 反応物 | Alpha-Ketoglutarate | HCID 164533 | — |
| 反応物 | l-aspartic acid, sodium salt | HCID 5460541 | — |
| 生成物 | Oxalacetate | HCID 164550 | — |
| 生成物 | L-glutamate(2-) | HCID 5460544 | — |
反応手順
PRINCIPLE ##STR5## AST catalyzes the transfer of the amino group from aspartate to 2-oxoglutarate to yield oxalacetate and glutamate. The oxalacetate is reduced to malate in the presence of malate dehydrogenase [MD] with the simultaneous oxidation of reduced nicotinamide adenine dinucleotide [NADH]. The rate of decrease in absorbance at 340 nm is directly proportional to AST activity.
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 特許番号 | US05382525 |
| 出典データセット | uspto-grants-1995_01 |
| ORD ID | ord-01a7a9d20ed64ae891a8cf0d3bd44d71 |
| 出典メモ | uspto-grants-1995_01 |