Thiodiglycol → Deoxycholic Acid

HRID 1813888

反応式

HRID 1813888 の反応式
C(O)CS[CH2:4][CH2:5][OH:6].CC(CC([C:16]1[CH:21]=[CH:20][C:19](OCCOCCO)=[CH:18][CH:17]=1)(C)C)(C)C.CCCCCCCCCCCC[O:41]CCO.[CH3:45][CH2:46][CH2:47][CH2:48][CH2:49][CH2:50][CH2:51][CH2:52][CH2:53][CH2:54][CH2:55][CH2:56][O:57][S:58]([O-:61])(=[O:60])=[O:59].[Na+:62].[CH3:63][C@@H:64]([C@@H:84]1[C@@:88]2(C)[C@@H:89]([OH:104])[CH2:90][C@@H:91]3[C@@:96]4([CH3:102])[CH2:97][CH2:98][C@@H](O)C[C@H:95]4C[C@@H](O)[C@H]3[C@@H:87]2CC1)[CH2:65][CH2:66][C:67](NCCC[N+](CC(O)CS([O-])(=O)=O)(C)C)=[O:68]>>[CH3:45][CH2:46][CH2:47][CH2:48][CH2:49][CH2:50][CH2:51][CH2:52][CH2:53][CH2:54][CH2:55][CH2:56][O:57][S:58]([O-:61])(=[O:60])=[O:59].[Na+:62].[CH3:63][C@@H:64]([C@@H:84]1[C@@:88]2([CH3:87])[C@@H:89]([OH:104])[CH2:90][C@@H:91]3[C@@:96]4([CH3:102])[CH2:97][CH2:98][C@@H:5]([OH:6])[CH2:4][C@H:95]4[CH2:18][CH2:17][C@H:16]3[C@@H:21]2[CH2:20][CH2:19]1)[CH2:65][CH2:66][C:67]([OH:68])=[O:41]

参加物

7

反応物 5

HCID 5447ThiodiglycolHCID 247502-(Dodecyloxy)ethanolHCID 24775Poly(oxy-1,2-ethanediyl), alpha-((1,1,3,3-tetramethylbutyl)phenyl)-omega-hydroxy-, potassium salt (1:1)HCID 1221453-((3-Cholamidopropyl)dimethylammonio)-2-hydroxy-1-propanesulfonateHCID 3423265Sodium Lauryl Sulfate

生成物 2

HCID 222528Deoxycholic AcidHCID 3423265Sodium Lauryl Sulfate

実験手順

The FIG. 7 shows the results. The samples were loaded on the gels in the following order (from left to right) lane-1 control sample, lane 2-3 thiodiglycol, lane 4-5 Tween-20, lane 6-7 NP-40, lane 8-9 Brij-35, lane 10 SDS, lane 11 CHAPSO, 12-13 Triton-X100, lane 14 CHAP lane 15 control. The electrophoresis results shows (after a comparison of several typical runs) that, with exception of SDS and de-oxycholate treated protein samples, most protein samples containing detergent showed batch to batch variation in protein precipitation yield. The protein precipitation yield depended on the techniques used for harvesting the precipitated protein. There were occasions when the precipitation yield of duplicate samples varied between 100% to 50%. Protein samples containing SDS invariably showed substantially 100% yield in multiple tests.

出典と根拠

ORDレコード
ord-2619c2e4fef642bd912dcf54c1e6b415
出典データセット
uspto-grants-2005_04
特許番号
US06875617B2

関連ノート

読み込み中…