Nadide (JAN/USAN/INN) → Propan-2-yl 4-bromo-3-hydroxybutanoate

HRID 1816192

反応式

HRID 1816192 の反応式
P(O)(O)([O-])=O.[K+].P(O)([O-])([O-])=O.[K+].[K+].C1C=[N+]([C@@H]2O[C@H](COP(OP(OC[C@H]3O[C@@H](N4C5N=CN=C(N)C=5N=C4)[C@H](O)[C@@H]3O)(O)=O)(O)=O)[C@@H](O)[C@H]2O)C=C(C(N)=O)C=1.CCCCCCCCCC.[Br:68][CH2:69][C:70](=[O:78])[CH2:71][C:72]([O:74][CH:75]([CH3:77])[CH3:76])=[O:73]>CC(O)C>[Br:68][CH2:69][CH:70]([OH:78])[CH2:71][C:72]([O:74][CH:75]([CH3:76])[CH3:77])=[O:73]

参加物

6

反応物 4

HCID 5893Nadide (JAN/USAN/INN)HCID 15600DecaneHCID 11075004Isopropyl 4-bromo-3-oxobutanoateHCID 21889592Monopotassium phosphate dipotassium phosphate

生成物 1

HCID 22492795Propan-2-yl 4-bromo-3-hydroxybutanoate

試薬・触媒・溶媒 1

HCID 3776Isopropanol溶媒

実験手順

To 50 ml of 50 mM monopotassium phosphate-dipotassium phosphate buffer (pH 7.0) containing 5% of 2-propanol were added 0.1 mmol of NAD+ and 6 g of the washed cells described above. To this mixture was added 50 ml of decane containing 70 mg (0.31 mmol) of isopropyl 4-bromo-3-oxobutanoate, and the mixture was stirred at room temperature for a night and day. Celite was then put into the reaction solution, and stirred for a while and filtered off to separate the resultant solution. The aqueous layer was further extracted three times with ethyl acetate, and combined organic layers were concentrated to give 50 mg of isopropyl 4-bromo-3-hydroxybutanoate.

後処理

  1. stirringstirred for a while
  2. filtrationfiltered off
  3. customto separate the resultant solution
  4. extractionThe aqueous layer was further extracted three times with ethyl acetate
  5. concentrationwere concentrated

出典と根拠

ORDレコード
ord-dd56711786f74bf99ae0757b88d9cd15
出典データセット
uspto-grants-2005_04
特許番号
US06884607B2

関連ノート

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