Phosphoric Acid → N-Carbamoylphenylalanine

HRID 1828670

反応式

HRID 1828670 の反応式
[CH:1]1[CH:6]=[CH:5][C:4]([CH2:7][CH:8]2[NH:14][C:12](=[O:13])[NH:11][C:9]2=[O:10])=[CH:3][CH:2]=1.[OH:15]P(O)(O)=O>[O-]S([O-])(=O)=O.[Cu+2]>[C:12]([NH:14][C@H:8]([C:9]([OH:15])=[O:10])[CH2:7][C:4]1[CH:5]=[CH:6][CH:1]=[CH:2][CH:3]=1)(=[O:13])[NH2:11]

参加物

4

反応物 2

HCID 1004Phosphoric AcidHCID 735655-(Phenylmethyl)-2,4-imidazolidinedione

生成物 1

HCID 446054N-Carbamoylphenylalanine

試薬・触媒・溶媒 1

HCID 24462Copper Sulfate触媒

実験手順

The assay of the D-hydantoinase activity was conducted by incubating a reaction mixture containing 120 mg/dl D-5-benzylhydantoin (BH), 50 mM KPB (pH 8.0) and an enzyme solution at 37° C. for 30 minutes, adding 9 volumes of 1.1 mM CuSO4, 11.1 mM H3PO4, centrifuging at 20,000 G for 10 minutes to remove the pellet, and then quantifying the resultant N-carbamylphenylalanine (N-Car-Phe) by HPLC. One unit of the enzyme activity was defined as an enzymatic activity capable of producing 1 μmol of N-carbamylphenylalanine per 1 minute under the condition described above.

後処理

  1. waitcentrifuging at 20,000 G for 10 minutes
  2. customto remove the pellet

出典と根拠

ORDレコード
ord-de44719ffd2e4d67b2f78cba251cc864
出典データセット
uspto-grants-2006_06
特許番号
US07060485B2

関連ノート

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