(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal → Ribosylthymidine diphosphate

HRID 1856592

反応式

HRID 1856592 の反応式
[P:1]([O:13][CH2:14][C@H:15]1[O:19][C@@H:18]([N:20]2[CH:27]=[C:26]([CH3:28])[C:24](=[O:25])[NH:23][C:21]2=[O:22])[C@H:17]([OH:29])[C@@H:16]1[OH:30])([O:4][P:5]([O:8]P(O)(O)=O)([OH:7])=[O:6])(=[O:3])[OH:2].O=C[C@@H]([C@H]([C@@H]([C@@H](CO)O)O)O)O.[O-]P(OP([O-])([O-])=O)(=O)[O-].[O-]P(OP(OP([O-])([O-])=O)([O-])=O)(=O)[O-]>C(O)C(N)(CO)CO.S([O-])([O-])(=O)=O.[NH4+].[NH4+]>[P:1]([O:13][CH2:14][C@H:15]1[O:19][C@@H:18]([N:20]2[CH:27]=[C:26]([CH3:28])[C:24](=[O:25])[NH:23][C:21]2=[O:22])[C@H:17]([OH:29])[C@@H:16]1[OH:30])([O:4][P:5]([OH:8])([OH:7])=[O:6])(=[O:2])[OH:3]

参加物

7

反応物 4

HCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanalHCID 4513885-Methyluridine 5'-triphosphateHCID 644102Pyrophosphate2 回HCID 3440921Triphosphate2 回

生成物 1

HCID 23379797Ribosylthymidine diphosphate

試薬・触媒・溶媒 2

HCID 6503Tris(hydroxymethyl)aminomethane溶媒2 回HCID 6097028Ammonium Sulfate溶媒

実験手順

5-Methyluridine 5'-triphosphate (0.0047 g, 0.009 mMol) was dissolved in 0.7 mL 1M Tris buffer (pH 8.5 containing 1M MgCl2) then 0.3 mL of a 0.25M glucose solution in 1M Tris buffer (pH 8.5 containing 1M MgCl2) added. A To timepoint was taken by reverse phase anion exchange HPLC. Hexokinase (EC 2.7.1.1, Boehringer Mannheim, from yeast overproducer), 75 units in 3.2M ammonium sulfate suspension, was added to the reaction which was incubated at 25° C. An HPLC was taken at 66 hours indicating complete conversion to diphosphate with no triphosphate remaining. Triphosphate retention: 17.5 minutes; diphosphate retention: 10.3 minutes.

後処理

  1. customwas incubated at 25° C
  2. custom17.5 minutes
  3. custom10.3 minutes

出典と根拠

ORDレコード
ord-6b74d2aa04b24b3ab82843ee73c9f71e
出典データセット
uspto-grants-2000_11
特許番号
US06143279

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