反応式
C(O)C(N)(CO)CO.CC(CC(C1C=[CH:21][C:20]([O:23]CCOCCO)=[CH:19][CH:18]=1)(C)C)(C)C.[F-].[Na+].C1(CS(F)(=O)=O)C=CC=CC=1.CC(C[C@H](NC(C)=O)C(N[C@H](C([NH:58][C@H:59]([C:67]([OH:69])=[O:68])[CH2:60][CH2:61][CH2:62]N=C(N)N)=O)CC(C)C)=O)C>OCC(CO)O>[NH2:58][C@H:59]([C:67]([OH:69])=[O:68])[CH2:60][C:61]1[CH:62]=[CH:21][C:20]([OH:23])=[CH:19][CH:18]=1参加物
7
実験手順
Sf21 cells were infected with a baculovirus expression vector for BTK or SYK, as previously described (refs 18 and 19). Cells were harvested, lysed (10 mM Tris pH 7.6, 100 mM NaCl, 1% Nonidet P-40, 10% glycerol, 50 mM NaF, 100 mM Na3VO4, 50 μg/ml phenylmethylsulfonyl fluoride, 10 μg/ml aprotonin, 10 μg/ml leupeptin), and the kinase proteins were purified to homogeneity, as reported (refs 18 and 19).
後処理
- customthe kinase proteins were purified to homogeneity
出典と根拠
- ORDレコード
- ord-6c1b4b0af82847228277616836d4b479
- 出典データセット
- uspto-grants-2002_05
- 特許番号
- US06391921B1
関連ノート
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