化工社化工社 化学情報印刷レポート · 研究参考用
Camp → Adenosine Monophosphate
関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|---|---|---|
| 反応物 | Camp | HCID 6076 | — |
| 反応物 | HCID 178418274 | HCID 178418274 | — |
| 生成物 | Adenosine Monophosphate | HCID 6083 | — |
| 生成物 | HCID 178418270 | HCID 178418270 | — |
反応手順
The enzyme of the present invention isolated and purified from rat cerebral cortex in Example was acted on [8-3H] cAMP and [8-3H] cGMP as substrates to form [8-3H] 5'-AMP and [8-3H] 5'-GMP, respectively. The products were reacted with 5'-nucleotidase to form [8-3H] adenosine and [8-3H] guanosine, respectively. These products were separated from each other on a cation exchange resin column (AG50 W-X4, Bio-Rad) to assay for the radioactivity. The assay was carried out in a manner similar to that of the enzymatic activity described in Example.
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 特許番号 | US05728563 |
| 出典データセット | uspto-grants-1998_03 |
| ORD ID | ord-4e4527ef977240138a93b4260c881157 |
| 出典メモ | uspto-grants-1998_03 |