To a mixture of N-(3-(dimethylamino)-1-{-4-[9-phenyl-3-(2-pyrimidinyl)[1,2,4]triazolo[3,4-f]-1,6-naphthyridin-8-yl]phenyl}cyclobutyl)-2,2,2-trifluoroacetamide (4-4) (10 mg, 0.016 mmol) in EtOH (1.0 mL) was added KOH (1M in water, 0.1 mL, 0.10 mmol), and the mixture was heated under microwave irradiation at 100° C. for 40 minutes. To the mixture was added EtOAc, sat. NaHCO3, and brine. The resulting solid was collected by filtration to give N3,N3-dimethyl-1-{4-[9-phenyl-3-(2-pyrimidinyl)-[1,2,4]triazolo[3,4-f]-1,6-naphthyridin-8-yl]phenyl}-1,3-cyclobutanediamine (4-5) as colorless solid. HRMS (M+H)+: observed=513.2515, calculated=513.2510.
