関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|
| 反応物 | Sodium Hydroxide | HCID 14798 | — |
| 反応物 | (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal | HCID 107526 | — |
| 反応物 | Biotin | HCID 171548 | — |
| 反応物 | Monobasic potassium phosphate | HCID 516951 | — |
| 反応物 | Ammonium Sulfate | HCID 6097028 | — |
| 反応物 | Thiaminhydrochloride | HCID 67590811 | — |
| 触媒 | Platinum | HCID 23939 | — |
| 生成物 | L-Lysine | HCID 5962 | — |
反応手順
A medium containing 45 g/L of glucose, 15 g/L of molasses, 2 g/L (as nitrogen) of soybean protein hydrolysate, 2 g/L of KH2PO4, 5.6 g/L of NaOH, 10 g/L of ammonium sulfate, 0.8 g/L of MgSO4.7H2O, 20 mg/L of FeSO4.7H2O, 20 mg/L of MnSO4.4H2O, 0.8 mg/L of thiamin hydrochloride and 0.2 mg/L of biotin (pH 6.0) was introduced into 1-L volume small glass fermentation tank in a volume of 300 mL and sterilized by heating at 120° C. for 20 minutes. After the fermentation tank was cooled to 31.5° C, 5 platinum loops of Brevibacterium lactofermentum ATCC 31269 (refer to U.S. Pat. No. 4,066,501) preliminarily grown on an LB plate for 24 hours was inoculated to the medium and cultured at 31.5° C. and pH 7.0 for 30 hours with sufficient aeration and stirring.
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|
| 特許番号 | US07416740B2 |
|---|
| 出典データセット | uspto-grants-2008_08 |
|---|
| ORD ID | ord-1b68ea4b5bc84dd5b31db6b4770dccc5 |
|---|
| 出典メモ | uspto-grants-2008_08 |
|---|
